WB (Western Blot)
(Western Blot analysis of A431 cells stimulated with pervanadate (1mM) for 30 minutes (lanes 1, 3, & 5) then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catanine , anti-β-Catenine (Tyr-489) conserved site , or anti-β-Catenine .)
gamma-Catenin Phospho-Regulation Antibody Sampler Kit
gamma-Catenin Phospho-Regulation
Applications
Immunoprecipitation, Immunocytochemistry, ELISA, Western Blot
Synonyms
gamma-Catenin Phospho-Regulation; N/A; Catenin 3K1; gamma-Catenin Phospho-Regulation antibody sampler kit
Form/Format
Supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for gamma-Catenin Phospho-Regulation antibody sampler kit
IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Preparation and Storage
Store at -20°C. Do not aliquot. Stable for 1 year.
WB (Western Blot)
(Western Blot analysis of A431 cells stimulated with pervanadate (1mM) for 30 minutes (lanes 1, 3, & 5) then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catanine , anti-β-Catenine (Tyr-489) conserved site , or anti-β-Catenine .)
WB (Western Blot)
(Western Blot analysis of A431 cells stimulated with pervanadate (1mM) for 30 minutes (lanes 1, 3, & 5) then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catanine , anti-β-Catenine (Tyr-489) conserved site , or anti-β-Catenine .)
WB (Western Blot)
(Westem blot analysis of γ-Catenin immunoprecipitated from A431 cells treated with pervanadate using anti-γ-Catenin (C-terminal). The immunoprecipitates were untreated (lanes 1, 3, & 7) or treated with alkaline phosphatase (lanes 2, 4, & 8). Blots of the immunoprecipitates were probed with anti-γ-Catenin (a.a. 545-555), anti-γ-Catenin (Tyr-550) or anti-γ-Catenin (C-terminal). In addition, the anti-γ-Catenin (Tyr-550) was used in the presence of phospho-γ-Catenin (Tyr-550) peptide (lane 5) or phospho-γ-Catenin (Tyr-644) peptide (lane6).)
WB (Western Blot)
(Westem blot analysis of γ-Catenin immunoprecipitated from A431 cells treated with pervanadate using anti-γ-Catenin (C-terminal). The immunoprecipitates were untreated (lanes 1, 3, & 7) or treated with alkaline phosphatase (lanes 2, 4, & 8). Blots of the immunoprecipitates were probed with anti-γ-Catenin (a.a. 545-555), anti-γ-Catenin (Tyr-550) or anti-γ-Catenin (C-terminal). In addition, the anti-γ-Catenin (Tyr-550) was used in the presence of phospho-γ-Catenin (Tyr-550) peptide (lane 5) or phospho-γ-Catenin (Tyr-644) peptide (lane6).)
Related Product Information for gamma-Catenin Phospho-Regulation antibody sampler kit
Background: Plakoglobin (gamma-catenin) is a catenin family member identified as a component of desmosomes. gamma-Catenin has high homology to beta-catenin and, like beta-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of alpha-catenin and at least one molecule of beta-catenin and gamma-catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of gamma-catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for gamma-catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues (Tyr-133, Tyr-480, Tyr-550, Tyr-644) in gamma-catenin can modify its interactions with other proteins. Phosphorylation of Tyr-644 decreases gamma-catenin association with alpha-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate Tyr-550, which increases gamma-catenin binding to alpha-catenin. Thus, tyrosine phosphorylation may be important for regulation of gamma-catenin protein-protein interactions within desmosomal complexes.
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Product Notes
The gamma-Catenin Phospho-Regulation (Catalog #AAA71572) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's gamma-Catenin Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the gamma-Catenin Phospho-Regulation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "gamma-Catenin Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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