LDH Cytotoxicity Assay Kit | LDH assay kit
LDH Cytotoxicity Assay Kit
Synonyms
LDH Cytotoxicity; N/A; LDH Cytotoxicity Assay Kit; LDH assay kit
Preparation and Storage
Store at -20 degree C and keep in dark. The kit has a storage time of 6 months from receipt.
Related Product Information for LDH assay kit
Principle of the Assay: Lactate dehydrogenase (L DH) is an oxidoreductase which cataly catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. LDH is a stable enzyme, present in all cell types, and rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study. LDH Cytotoxicity Assay Kit provides a simple and easy colorimetric assay for the study of cytotoxicity. This assay is based on in a typical cytotoxicity assay, target ce lls are cultured with a cytotoxic chemical agent or a cytotoxic cell (NK cell, cytotoxic T cells) to induce target cell death and LDH release. The LDH LDH-containing supernatants are transferred to wells of a new 96 96-well assay plate and mixed with the LDH Reac tion solution. On the action of LDH, NADH reacts with the tetrazolium salt MTT to generate the reduced form of NAD NAD+ and MTT. The reduced form of MTT exhibits a maximum absorption at 565 nm. The intensity of the generated color correlates directly with the cell number lysed. After an incubation of 30 min at room temperature, the absorbance at 565 nm is read using a plate reader.
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Product Notes
The LDH (Catalog #AAA330128) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "LDH Cytotoxicity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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