CTLA-4 active protein

Recombinant CTLA-4

Cat. Num. AAA48326

• Gene Names: CTLA4; CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
• Applications: SDS-Page
• Purity: > 90% by SDS - PAGE
• Synonyms: CTLA-4; N/A; Recombinant CTLA-4; CTLA4; Cytotoxic T-lymphocyte-associated protein 4; Cytotoxic T-lymphocyte-associated antigen 4; ALPS5; CD; CD152; CELIAC3; GRD4; GSE; IDDM12; CTLA-4 active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS - PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 36-161aa
• Sequence: KAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMG NELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVI DPEPCPDSD
• Sequence Length: 223

• Applicable Applications for CTLA-4 active protein: Bioactivity, SDS-PAGE
• Tag: His-Tag
• Biological Activity: The activity is determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells. The ED50 range 150 ng/ml with Human B7-1/CD80.
• Predicted nterm: Ala
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human CTLA-4 inhibits Human B7-1/CD80 induced IL-2 secretion of the Jurkat human acute T cell leukemia cells. The ED50 range 150 ng/ml.)

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for CTLA-4 active protein

CTLA-4, also known as cytotoxic T-lymphocyte protein 4, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells. It also is a protein receptor that functions as an immune checkpoint and downregulates immune responses. This protein is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation a phenomenon which is particularly notable in cancers. It may also function via modulation of cell motility and signaling through PI3 kinase. It has been implicated in several autoimmune diseases, such as systemic lupus erythematosus. Recombinant human CTLA-4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CTLA-4 active protein

Immunology

References

Dai F., et al. (2015) Braz J Med Biol Res. 48:629-636. ;;Ward FJ., et al. (2013) Eur J Immunol. 43:1274-1285.

NCBI and Uniprot Product Information

• NCBI GI #: 27735177
• NCBI GeneID: 1493
• UniProt Accession #: P16410
• Molecular Weight: 14.6kDa (135aa)
• NCBI Official Full Name: Cytotoxic T-lymphocyte protein 4
• NCBI Official Synonym Full Names: cytotoxic T-lymphocyte associated protein 4
• NCBI Official Symbol: CTLA4
• NCBI Official Synonym Symbols: CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
• NCBI Protein Information: cytotoxic T-lymphocyte protein 4
• UniProt Protein Name: Cytotoxic T-lymphocyte protein 4
• UniProt Gene Name: CTLA4
• UniProt Synonym Gene Names: CD152; CTLA-4
• UniProt Entry Name: CTLA4_HUMAN