Application Data
(Figure 3. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein 1ug/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.)
CTLA-4 recombinant protein
Recombinant human CTLA-4 protein with C-terminal human Fc tag
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
CTLA-4; N/A; Recombinant human CTLA-4 protein with C-terminal human Fc tag; CTLA4; CD152; CTLA-4 recombinant protein
Host
HEK293
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5 %-8% trehalose is added as protectants before lyophilization.
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Application Data
(Figure 3. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein 1ug/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.)
Application Data
(Figure 3. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein 1ug/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.)
ELISA
(Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CTLA-4, hFc tagged protein can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.64-80.0ng/ml.)
ELISA
(Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CTLA-4, hFc tagged protein can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.64-80.0ng/ml.)
SDS-PAGE
(Figure 1. Human CTLA-4 Protein, hFc Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human CTLA-4 Protein, hFc Tag on SDS-PAGE under reducing condition.)
Related Product Information for CTLA-4 recombinant protein
The protein has a predicted molecular mass of 39.6 kDa after removal of the signal peptide. The apparent molecular mass of CTLA4-hFC is approximately 40-55 kDa due to glycosylation. This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Product Categories/Family for CTLA-4 recombinant protein
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Product Notes
The CTLA-4 (Catalog #AAA78604) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CTLA-4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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