Bioactivity
(Mouse Ctla4 inhibits IL-2 secretion of the stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range )
Ctla-4 active protein
Recombinant mouse Ctla-4 protein
Gene Names
Ctla4; Cd152; Ly-56; Ctla-4
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
Ctla-4; N/A; Recombinant mouse Ctla-4 protein; cytotoxic T-lymphocyte protein 4 isoform 1; Ly-5; Ctla; Cytotoxic T-lymphocyte-associated antigen 4; CTLA4; Cd152; Ly-56; Ctla4; Ctla-4 active protein
Host
Baculovirus
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
38-161aa
Sequence
IQVTQPSVVL ASSHGVASFP CEYSPSHNTD EVRVTVLRQT NDQMTEVCAT TFTEKNTVGF LDYPFCSGTF NESRVNLTIQ GLRAVDTGLY LCKVELMYPP PYFVGMGNGT QIYVIDPEPC PDSD
Applicable Applications for Ctla-4 active protein
Bioactivity, SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Mouse
Tag
hIgG-His-Tag
Predicted N-term
Ile 38
Bioactivity
The activity is determined by the IL-2 ELISA in a using stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range <=150ng/ml.
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
Bioactivity
(Mouse Ctla4 inhibits IL-2 secretion of the stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range )
Bioactivity
(Mouse Ctla4 inhibits IL-2 secretion of the stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range )
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for Ctla-4 active protein
Ctla-4, also known as cytotoxic T-lymphocyte protein 4 isoform 1, is a type 1 membrane protein and a memberof the immunoglobulin-superfamily. Besides, this protein contains an extra cellular domain, a transmembranedomain and a cytoplasmic tail. It is generally expressed with highest levels in lymphoid tissues. Also, it is similarto T-cell costimulatory protein CD28 since both of the molecules bind to CD80 and CD86 on antigen-presenting cells. CTLA4 and CD28 effectively regulate to T cell responses as opposing signals transmitted through tworelated cell-surface receptors. Recombinant mouse Ctla-4, fused to hIgG-His-tag at C-terminus, was expressed ininsect cell and purified by using conventional chromatography techniques.
Product Categories/Family for Ctla-4 active protein
References
Krummey SM., et al. (2014) J. Immunol. 192:2495-2504. Jain N., et al. (2013) Nat. Med. 19:1632-1637. Yu CR., et al. (2013) J. Immunol. 191:5036-5043.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,993 Da
NCBI Official Full Name
cytotoxic T-lymphocyte protein 4 isoform 1
NCBI Official Synonym Full Names
cytotoxic T-lymphocyte-associated protein 4
NCBI Official Symbol
Ctla4
NCBI Official Synonym Symbols
Cd152; Ly-56; Ctla-4
NCBI Protein Information
cytotoxic T-lymphocyte protein 4; CD152 antigen; cytotoxic T-lymphocyte-associated antigen 4
UniProt Protein Name
Cytotoxic T-lymphocyte protein 4
UniProt Gene Name
Ctla4
UniProt Synonym Gene Names
Cd152; CTLA-4
UniProt Entry Name
CTLA4_MOUSE
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Product Notes
The Ctla-4 ctla4 (Catalog #AAA48408) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 38-161aa. AAA Biotech's Ctla-4 can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the Ctla-4 ctla4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: IQVTQPSVVL ASSHGVASFP CEYSPSHNTD EVRVTVLRQT NDQMTEVCAT TFTEKNTVGF LDYPFCSGTF NESRVNLTIQ GLRAVDTGLY LCKVELMYPP PYFVGMGNGT QIYVIDPEPC PDSDPrecautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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