Bioactivity
(ADP-Glo assay for SMARCA4 (658-1328) activity (Data from ICE Bioscience Inc.) 100 uM ATP and 10 nM DNA was incubated with different concentrations of SMARCA4 (658-1328) protein in a 10 ul reaction system containing 20 mM HEPES pH 7.5,10 mM MgCl2, 50 mM NaCl, 0.1%Tween-20, 1 mM DTT for 1 hour, 10 ul ADP-Glo Reagent was added to the products and incubated for 1 hour. Then 20 ul Kinase Detection Reagent incubated for 1 hour. All the operations and reactions were performed at RT. Luminescence measurement is collected by BMG.)
SMARCA4 active protein
Recombinant SMARCA4 (658-1328) protein
Synonyms
SMARCA4; N/A; Recombinant SMARCA4 (658-1328) protein; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SMARCA4 active protein
Host
Baculovirus
Form/Format
Recombinant SMARCA4 (658-1328) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Source
Human
Tag
N terminal DYKDDDDK-Tag
Protein Details
Recombinant SMARCA4 protein that includes amino acids 658-1328 of human SMARCA4 protein (accession number NP_001122317.1) was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag. The molecular weight of the protein is 78.8 kDa.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. T
Bioactivity
(ADP-Glo assay for SMARCA4 (658-1328) activity (Data from ICE Bioscience Inc.) 100 uM ATP and 10 nM DNA was incubated with different concentrations of SMARCA4 (658-1328) protein in a 10 ul reaction system containing 20 mM HEPES pH 7.5,10 mM MgCl2, 50 mM NaCl, 0.1%Tween-20, 1 mM DTT for 1 hour, 10 ul ADP-Glo Reagent was added to the products and incubated for 1 hour. Then 20 ul Kinase Detection Reagent incubated for 1 hour. All the operations and reactions were performed at RT. Luminescence measurement is collected by BMG.)
Bioactivity
(ADP-Glo assay for SMARCA4 (658-1328) activity (Data from ICE Bioscience Inc.) 100 uM ATP and 10 nM DNA was incubated with different concentrations of SMARCA4 (658-1328) protein in a 10 ul reaction system containing 20 mM HEPES pH 7.5,10 mM MgCl2, 50 mM NaCl, 0.1%Tween-20, 1 mM DTT for 1 hour, 10 ul ADP-Glo Reagent was added to the products and incubated for 1 hour. Then 20 ul Kinase Detection Reagent incubated for 1 hour. All the operations and reactions were performed at RT. Luminescence measurement is collected by BMG.)
SDS-PAGE
(Recombinant SMARCA4 (658-1328) protein 10% SDS-PAGE Coomassie staining MW: 78.8 kDa Purity: >85%)
SDS-PAGE
(Recombinant SMARCA4 (658-1328) protein 10% SDS-PAGE Coomassie staining MW: 78.8 kDa Purity: >85%)
Related Product Information for SMARCA4 active protein
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA4 contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA4 is part of the large ATP-dependent chromatin remodeling complex SNF/SWI which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Gene mutation causes Rhabdoid Tumor Predisposition Syndrome Type 2. SMARCA4 functions as a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It also interacts with glucocorticoid receptor (GR), TOPBP1 and progesterone receptor (PR) and is a component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Somatic mutations of SMARCA4 have been detected in some cancer cell lines and loss of SMARCA4 is associated with decreased survival in cancer patients. It shows binding specificity for acetylated H2BK5, H3K14 and H3K9.
Product Categories/Family for SMARCA4 active protein
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Product Notes
The SMARCA4 (Catalog #AAA60347) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SMARCA4, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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