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product-image-AAA40772_IF8.gif IF (Immunofluorescence) (Immunofluorescence of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 20 μg/ml.Green: SMARCA4 Antibody (7749)Blue: DAPI staining)

Rabbit SMARCA4 Polyclonal Antibody | anti-SMARCA4 antibody

SMARCA4 Antibody

Gene Names
SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
SMARCA4 antibody is affinity chromatography purified via peptide column.
Synonyms
SMARCA4, Antibody; SMARCA4 Antibody; SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2B; Transcription activator BRG1; ATP-dependent helicase SMARCA4; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; anti-SMARCA4 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SMARCA4 antibody is human, mouse and rat reactive. Multiple isoforms of SMARCA4 are known to exist.
Purity/Purification
SMARCA4 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1679
Applicable Applications for anti-SMARCA4 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
SMARCA4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4.
Buffer
SMARCA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SMARCA4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

IF (Immunofluorescence)

(Immunofluorescence of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 20 μg/ml.Green: SMARCA4 Antibody (7749)Blue: DAPI staining)

product-image-AAA40772_IF8.gif IF (Immunofluorescence) (Immunofluorescence of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 20 μg/ml.Green: SMARCA4 Antibody (7749)Blue: DAPI staining)

IHC (Immunohistochemistry)

(Immunohistochemistry of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 2 μg/ml.)

product-image-AAA40772_IHC10.gif IHC (Immunohistochemistry) (Immunohistochemistry of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 2 μg/ml.)

IF (Immunofluorescence)

(Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.)

product-image-AAA40772_IF11.jpg IF (Immunofluorescence) (Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.)

product-image-AAA40772_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 μg/ml.)

product-image-AAA40772_WB15.gif WB (Western Blot) (Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 μg/ml.)
Related Product Information for anti-SMARCA4 antibody
The SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (SMARCA4) protein, also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila (1). Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes (2). The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (3). In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44 (4,5).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 185 kDa

Observed: 200 kDa
NCBI Official Full Name
transcription activator BRG1 isoform A
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
NCBI Official Symbol
SMARCA4
NCBI Official Synonym Symbols
BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
NCBI Protein Information
transcription activator BRG1; SNF2-beta; SNF2-like 4; protein BRG-1; nuclear protein GRB1; brahma protein-like 1; BRM/SWI2-related gene 1; homeotic gene regulator; protein brahma homolog 1; BRG1-associated factor 190A; sucrose nonfermenting-like 4; ATP-dependent helicase SMARCA4; mitotic growth and transcription activator; global transcription activator homologous sequence
UniProt Protein Name
Transcription activator BRG1
UniProt Gene Name
SMARCA4
UniProt Synonym Gene Names
BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
UniProt Entry Name
SMCA4_HUMAN

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Product Notes

The SMARCA4 smarca4 (Catalog #AAA40772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMARCA4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA4 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SMARCA4 smarca4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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