Canine MMP-9 (Matrix Metalloproteinase 9) ELISA Kit | MMP-9 elisa kit

Canine MMP-9 (Matrix Metalloproteinase 9) ELISA Kit

Cat. Num. AAA249557

• Reactivity: Canine
• Synonyms: MMP-9 (Matrix Metalloproteinase 9); N/A; Canine MMP-9 (Matrix Metalloproteinase 9) ELISA Kit; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; MMP9; MMP-9 elisa kit

• Reactivity: Canine
• Specificity: Specifically recognize MMP-9, no obvious cross reaction with other analogues
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for MMP-9 elisa kit

Background: Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes.MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti MMP-9 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti MMP-9 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with MMP-9 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of MMP-9 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 50978992
• NCBI GeneID: 403885
• NCBI Accession #: NP_001003219.1
• NCBI GenBank Nucleotide #: NM_001003219.2
• UniProt Accession #: O18733; O19130
• Molecular Weight: 78,123 Da
• NCBI Official Full Name: matrix metalloproteinase-9
• NCBI Official Symbol: MMP9
• NCBI Protein Information: matrix metalloproteinase-9; GELB; MMP-9; gelatinase B; 92 kDa gelatinase; type IV collagenase MMP-9; 92 kDa type IV collagenase; matrix metalloproteinase 9
• UniProt Protein Name: Matrix metalloproteinase-9
• UniProt Gene Name: MMP9
• UniProt Synonym Gene Names: MMP-9; GELB
• UniProt Entry Name: MMP9_CANFA