SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
CD47 recombinant protein
CD47, 19-141aa, Human, hIgG-His tag, Baculovirus
Gene Names
CD47; IAP; OA3; MER6
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
CD47; N/A; CD47, 19-141aa, Human, hIgG-His tag, Baculovirus; Leukocyte surface antigen CD47 isoform 1; IAP; MER6; OA3; CD47 recombinant protein
Host
Baculovirus
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNELEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH
Sequence Length
323
Applicable Applications for CD47 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
hIgG-His-tag
Domain
19-141aa
Endotoxin Level
< 1 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at +2°C to +8°C for one week, For long term storage , aliqout and store at -20°C to -80°C.
Avoid repeated freezing thawing cycles.
Avoid repeated freezing thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for CD47 recombinant protein
CD47, also known as leukocyte surface antigen CD47 isoform 1, is a member of immunoglobulin superfamily. It has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Also, it plays an important role in memory formation and synaptic plasticity in the hippocampus. This protein is a receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 mediates cell-cell adhesion, enhances super antigen-dependent T-cell-mediated proliferation and co-stimulates T-cell activation. It may play a role in membrane transport and/or integrin dependent signal transduction and prevent premature elimination of red blood cells. Recombinant human CD47, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for CD47 recombinant protein
References
Iwamoto C., et al. (2014) Exp. Hematol. 42:163-171.; Wang Y., et al. (2013) Mol. Ther. 21:1919-1929.; Jiang H., et al. (2013) Leuk. Res. 37:907-910.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 66.38 kDa
NCBI Official Full Name
leukocyte surface antigen CD47 isoform 1
NCBI Official Synonym Full Names
CD47 molecule
NCBI Official Symbol
CD47
NCBI Official Synonym Symbols
IAP; OA3; MER6
NCBI Protein Information
leukocyte surface antigen CD47
UniProt Protein Name
Leukocyte surface antigen CD47
UniProt Gene Name
CD47
UniProt Synonym Gene Names
MER6; IAP
Similar Products
Product Notes
The CD47 cd47 (Catalog #AAA48923) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is hIgG-His-tag!!Domain||19-141aa. AAA Biotech's CD47 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CD47 cd47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNELEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH. It is sometimes possible for the material contained within the vial of "CD47, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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