SDS-PAGE
Catenin beta Recombinant Protein | CTNNB1 recombinant protein
Catenin beta recombinant protein
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Applications
Western Blot
Purity
>95%
Synonyms
Catenin beta; N/A; Catenin beta recombinant protein; CTNNB; FLJ25606; Catenin b Protein; CTNNB1 recombinant protein
Host
Sf9 insect cells
Purity/Purification
>95%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.2 ug/ul (varies by lot)
Sequence Length
3720
Applicable Applications for CTNNB1 recombinant protein
WB (Western Blot)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human Catenin beta was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for CTNNB1 recombinant protein
Recombinant full-length human Catenin beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is (NM_001904)
Scientific Background: beta-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins associate with E-cadherin at cellular junctions (1). beta-catenin interacts with TCF and LEF transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway. The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and GSK3beta, form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of beta-catenin by the proteasome. APC and Siah-1 mediate a novel beta-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas (2).
Scientific Background: beta-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins associate with E-cadherin at cellular junctions (1). beta-catenin interacts with TCF and LEF transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway. The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and GSK3beta, form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of beta-catenin by the proteasome. APC and Siah-1 mediate a novel beta-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas (2).
Product Categories/Family for CTNNB1 recombinant protein
References
1. Morin, P.J. et al: Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Science 1997; 275(5307):1787-1790.
2. Liu, J. et al: Siah-1 mediates a novel beta-catenin degradation pathway linking p53 to the adenomatous polyposis coli protein. Mol. Cell. 2001;7(5):927-36.
2. Liu, J. et al: Siah-1 mediates a novel beta-catenin degradation pathway linking p53 to the adenomatous polyposis coli protein. Mol. Cell. 2001;7(5):927-36.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~115kDa
NCBI Official Full Name
Homo sapiens catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), beta 1, 88kDa
NCBI Official Symbol
CTNNB1
NCBI Official Synonym Symbols
CTNNB; MRD19; armadillo
NCBI Protein Information
catenin beta-1
UniProt Protein Name
Catenin beta-1
UniProt Gene Name
CTNNB1
UniProt Synonym Gene Names
CTNNB
UniProt Entry Name
CTNB1_HUMAN
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Product Notes
The CTNNB1 ctnnb1 (Catalog #AAA72898) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human Catenin beta was expressed by baculovirus in Sf9 insect cells. AAA Biotech's Catenin beta can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CTNNB1 ctnnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Catenin beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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