ApoScreen Annexin V Apoptosis Kit FITC Kit | ANXA5 fitc kit

Aposcreen Annexin V Apoptosis Kit-FITC

Cat. Num. AAA78882

• Gene Names: ANXA5; PP4; ANX5; ENX2
• Applications: Flow Cytometry, Functional Assay
• Synonyms: ApoScreen Annexin V Apoptosis Kit; N/A; Aposcreen Annexin V Apoptosis Kit-FITC; Aposcreen Annexin V Apoptosis Kit - FITC; ANXA5 fitc kit

• Sequence Length: 320

• Applicable Applications for ANXA5 fitc kit: FCM/FACS (Flow Cytometry)
• Kit Components and Storage Handling: MBS678004_A : Annexin V-FITC is supplied as 100 tests in 1.0 mL of Annexin V Binding Buffer. Store at 2-8°C**.; MBS678004_B : Propidium Iodide (PI) is supplied as 100 tests in 1.0 mL of Annexin V Binding Buffer. Store at 2-8°C**.; MBS678004_C : Annexin V Binding Buffer 10X is supplied as 7 x 1.5 mL. Store at 2-8°C.; **Protect FITC conjugated and PI reagents from light. Reagents are stable for the period shown on the label if stored as directed.

FCM/FACS (Flow Cytometry)

(Day old BALB/c mouse splenocytes were stained with ApoScreen® Annexin V Apoptosis Kit-FITC (AAA78882))

Related Product Information for ANXA5 fitc kit

ApoScreen® Annexin V Apoptosis Kit-FITC (Cat. No. MBS678004) employs a fluorescein-conjugated Annexin V (Annexin V-FITC) in concert with propidium iodide (PI) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis, cells begin to display phosphatidylserine (PS) on the outer cell membrane where it is readily detectable by staining the cells with Annexin V-FITC. As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, PI can readily move across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lost membrane integrity through mechanisms including necrosis. When cells are double stained with Annexin V-FITC and PI, three different cell populations may be observed - (i) live cells that do not stain with either Annexin V-FITC or PI; (ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with Annexin V-FITC only. Analysis may be performed on any flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells can be examined with a fluorescence microscope equipped with filters for discrimination of FITC and rhodamine/PI.

Product Categories/Family for ANXA5 fitc kit

ApoScreen Annexin V Kit

References

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NCBI and Uniprot Product Information

• NCBI GI #: 4502107
• NCBI GeneID: 308
• NCBI Accession #: NP_001145.1
• NCBI GenBank Nucleotide #: NM_001154.3
• UniProt Accession #: P08758; Q6FHB3; Q6FI16; Q8WV69; Q9UDH9; D3DNW7
• NCBI Official Full Name: annexin A5
• NCBI Official Synonym Full Names: annexin A5
• NCBI Official Symbol: ANXA5
• NCBI Official Synonym Symbols: PP4; ANX5; ENX2
• NCBI Protein Information: annexin A5; CBP-I; PAP-I; VAC-alpha; annexin V; annexin-5; anchorin CII; endonexin II; lipocortin V; calphobindin I; OTTHUMP00000164026; OTTHUMP00000219982; OTTHUMP00000219983; OTTHUMP00000219985; thromboplastin inhibitor; vascular anticoagulant-alpha; placental anticoagulant protein 4; placental anticoagulant protein I
• UniProt Protein Name: Annexin A5
• UniProt Gene Name: ANXA5
• UniProt Synonym Gene Names: ANX5; ENX2; PP4
• UniProt Entry Name: ANXA5_HUMAN