DNase I Active Protein | DNASE1 active protein

DNase I Protein, Human, Recombinant (His Tag)

Cat. Num. AAA258026

• Gene Names: DNASE1; DNL1; DRNI
• Purity: >85% as determined by SDS-PAGE
• Synonyms: DNase I; N/A; DNase I Protein, Human, Recombinant (His Tag); Human DNASE1/Deoxyribonuclease I/DNL1 Protein (His Tag); DNL1 Protein, Human; DRNI Protein, Human; deoxyribonuclease I; DNASE1 active protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4, 5% trehalose, 5% mannitol and 0.01% Tween80
• Sequence: Met1-Lys282

• Species: Human
• Bioactivity: One unit is defined as the amount of DNase I that degrades DNA and causes an increase in absorbance at 260 nm of 0.001/minute at 25°C, pH5.0. The specific activity is >5000unit/mg.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human DNASE1 (P24855)(Met1-Lys282) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV
• Stability: Samples are stable for up to 12 months from date of receipt at -70°C
• Preparation and Storage: Store under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for DNASE1 active protein

Background: DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.

References

Shak S. et al., 1991, Proc Natl Acad Sci. 87 (23): 9188-92. Yasutomo K. et al., 2001, Nat Genet. 28 (4): 313-4. Hakkim A. et al., 2010, Proc Natl Acad Sci. 107 (21): 9813-8.

NCBI and Uniprot Product Information

• NCBI GI #: 118919
• NCBI GeneID: 1773
• UniProt Accession #: P24855; Q14UU9; Q14UV0; B4DV35
• Molecular Weight: The recombinant human DNASE1 consists of 271 amino acids and predicts a molecular mass of 30.7 kDa. It migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: Deoxyribonuclease-1
• NCBI Official Synonym Full Names: deoxyribonuclease I
• NCBI Official Symbol: DNASE1
• NCBI Official Synonym Symbols: DNL1; DRNI
• NCBI Protein Information: deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I
• UniProt Protein Name: Deoxyribonuclease-1
• UniProt Gene Name: DNASE1
• UniProt Synonym Gene Names: DNL1; DRNI; DNase I
• UniProt Entry Name: DNAS1_HUMAN