XEDAR / EDA2R recombinant protein

Recombinant Human XEDAR / EDA2R Protein (His tag)

Cat. Num. AAA173539

• Gene Names: EDA2R; XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: XEDAR / EDA2R; N/A; Recombinant Human XEDAR / EDA2R Protein (His tag); UNQ2448 / PRO5727 / PRO34080, EDA-A2R, EDAA2R, TNFRSF27, XEDAR; XEDAR / EDA2R recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80
• Sequence Length: 297

• Expression Host: Met 1-Thr 138
• Tag: C-His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Reconstitution: We recommend centrifuging the vial before opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Concentration is measured by UV-Vis
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 degree C to -80 degree C.; Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months

SDS-PAGE

Related Product Information for XEDAR / EDA2R recombinant protein

Background: Tumor necrosis factor receptor superfamily member 27, also known as X-linked ectodysplasin-A2 receptor, EDA-A2 receptor, EDA2R, XEDAR and TNFRSF27, is a single-pass type I II membrane protein. TNFRSF27 / EDA2R contains three TNFR-Cys repeats. It is a new member of the tumor necrosis factor receptor family that has been shown to be highly expressed in ectodermal derivatives during embryonic development and binds to ectodysplasin-A2 (EDA-A2). TNFRSF27 / EDA2R is a receptor for EDA isoform A2, but not for EDA isoform A1. TNFRSF27 / EDA2R mediates the activation of the NF-kappa-B and JNK pathways. The activation seems to be mediated by binding to TRAF3 and TRAF6. Ectodysplasin, a member of the tumor necrosis factor family, is encoded by the anhidrotic ectodermal dysplasia EDA gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that differ only by an insertion of two amino acids. This insertion functions to determine receptor binding specificity, such that EDA-A1 binds only the receptor EDAR, whereas EDA-A2 binds only the related, but distinct, X-linked ectodysplasin-A2 receptor (XEDAR).

Description: A DNA sequence encoding the human EDA2R (NP_068555.1) extracellular domain (Met 1-Thr 138) fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 11140823
• NCBI GeneID: 60401
• NCBI Accession #: NP_068555.1
• NCBI GenBank Nucleotide #: NM_021783.4
• UniProt Accession #: Q9HAV5; Q5VYX9; Q5VYY0; Q6UWM2; Q8IZA6
• Molecular Weight: Calculated: 16.7 kDa; Obeserved: 33 kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 27 isoform 1
• NCBI Official Synonym Full Names: ectodysplasin A2 receptor
• NCBI Official Symbol: EDA2R
• NCBI Official Synonym Symbols: XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 27
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 27
• UniProt Gene Name: EDA2R
• UniProt Synonym Gene Names: TNFRSF27; XEDAR; EDA-A2 receptor