Application Data
(Recombinant EHMT2 (G9A)-SET (913-1193) activity assay. 3.3 uM H3 (1-21) peptide was incubated with EHMT2 (G9A)-SET (913-1193 protein in reaction buffer including 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours at room temperature. H3K9me2 antibody is used to detect reaction product. )
EHMT2 recombinant protein
Recombinant EHMT2 (G9A)-SET (913-1193) protein
Gene Names
EHMT2; G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
Synonyms
EHMT2; N/A; Recombinant EHMT2 (G9A)-SET (913-1193) protein; BAT8; GAT8; NG36; KMT1C; C6orf30; EHMT2 recombinant protein
Host
E Coli
Form/Format
Recombinant EHMT2 (G9A)-SET (913-1193) protein expressed in E Coli and supplied in 25mM Tris-HCl pH8.0, 300mM NaCl and 5% glycerol.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant EHMT2 (G9A)-SET (913-1193) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant EHMT2 (G9A)-SET (913-1193) activity assay. 3.3 uM H3 (1-21) peptide was incubated with EHMT2 (G9A)-SET (913-1193 protein in reaction buffer including 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours at room temperature. H3K9me2 antibody is used to detect reaction product. )
Application Data
(Recombinant EHMT2 (G9A)-SET (913-1193) activity assay. 3.3 uM H3 (1-21) peptide was incubated with EHMT2 (G9A)-SET (913-1193 protein in reaction buffer including 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours at room temperature. H3K9me2 antibody is used to detect reaction product. )
SDS-PAGE
(Recombinant EHMT2 (G9A)-SET (913-1193) protein gel. EHMT2 (G9A)-SET (913-1193) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE
(Recombinant EHMT2 (G9A)-SET (913-1193) protein gel. EHMT2 (G9A)-SET (913-1193) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
Related Product Information for EHMT2 recombinant protein
Short Description: The peptide corresponding to amino acids 913-1193 of the G9a protein (accession number NP_006700.3) was expressed in E Coli. The peptide contains the SET domain of G9a that is responsible for methyltransferase activity. Recombinant G9a-SET contains N-terminal GST-Tag with an observed molecular weight of 58.7 kDa. G9a, also known as Euchromatic Histone-lysine N-methyltransferase 2 (EHMT2), is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. G9a also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. G9a also weakly methylates Lys27 of histone H3 (H3K27me). In addition, G9a is required for DNA methylation. However, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. G9a is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a may also methylate histone H1. In addition to histone methyltransferase activity, G9a also methylates non-histone proteins. It has been shown to mediate dimethylation of Lys373 of p53 and also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. Recombinant G9a-SET can be used for enzyme kinetics and inhibitor screening studies.
Background: EHMT2 (G9A)-SET, also known as Euchromatic Histone-lysine N-methyltransferase 2 (EHMT2), is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. EHMT2 (G9A)-SET also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. G9a also weakly methylates Lys27 of histone H3 (H3K27me). In addition, G9a is required for DNA methylation. However, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. G9a is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a may also methylate histone H1. In addition to histone methyltransferase activity, G9a also methylates non-histone proteins. It has been shown to mediate dimethylation of Lys373 of p53 and also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
Background: EHMT2 (G9A)-SET, also known as Euchromatic Histone-lysine N-methyltransferase 2 (EHMT2), is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. EHMT2 (G9A)-SET also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. G9a also weakly methylates Lys27 of histone H3 (H3K27me). In addition, G9a is required for DNA methylation. However, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. G9a is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a may also methylate histone H1. In addition to histone methyltransferase activity, G9a also methylates non-histone proteins. It has been shown to mediate dimethylation of Lys373 of p53 and also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
Product Categories/Family for EHMT2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
132370
NCBI Official Full Name
histone-lysine N-methyltransferase EHMT2 isoform a
NCBI Official Synonym Full Names
euchromatic histone-lysine N-methyltransferase 2
NCBI Official Symbol
EHMT2
NCBI Official Synonym Symbols
G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
NCBI Protein Information
histone-lysine N-methyltransferase EHMT2
UniProt Protein Name
Histone-lysine N-methyltransferase EHMT2
UniProt Gene Name
EHMT2
UniProt Synonym Gene Names
BAT8; C6orf30; G9A; KMT1C; NG36; H3-K9-HMTase 3
UniProt Entry Name
EHMT2_HUMAN
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Product Notes
The EHMT2 ehmt2 (Catalog #AAA59675) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EHMT2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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