WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using EHMT2 antibody.)
Rabbit EHMT2 Polyclonal Antibody | anti-EHMT2 antibody
EHMT2 Antibody
Gene Names
EHMT2; G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
EHMT2, Antibody; EHMT2 Antibody; EHMT2; BAT8; C6orf30; DKFZp686H08213; FLJ35547; anti-EHMT2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total EHMT2 protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
1233
Applicable Applications for anti-EHMT2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human EHMT2.
Target Name
EHMT2
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using EHMT2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using EHMT2 antibody.)
Related Product Information for anti-EHMT2 antibody
G9a, also known as Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1,2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3,4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-dimerization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large transcriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12).
Product Categories/Family for anti-EHMT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
132kd
NCBI Official Full Name
histone-lysine N-methyltransferase EHMT2 isoform c
NCBI Official Synonym Full Names
euchromatic histone-lysine N-methyltransferase 2
NCBI Official Symbol
EHMT2
NCBI Official Synonym Symbols
G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
NCBI Protein Information
histone-lysine N-methyltransferase EHMT2
UniProt Protein Name
Histone-lysine N-methyltransferase EHMT2
UniProt Gene Name
EHMT2
UniProt Synonym Gene Names
BAT8; C6orf30; G9A; KMT1C; NG36; H3-K9-HMTase 3
UniProt Entry Name
EHMT2_HUMAN
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Product Notes
The EHMT2 ehmt2 (Catalog #AAA302786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EHMT2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EHMT2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the EHMT2 ehmt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EHMT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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