WB (Western Blot)
(Detection of human G9A/EHMT2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody AAA211232 (lot AAA211232-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human G9A/EHMT2 Polyclonal Antibody | anti-G9A/EHMT2 antibody
Rabbit anti-G9A/EHMT2 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
G9A/EHMT2, Antibody; Rabbit anti-G9A/EHMT2 Antibody, Affinity Purified; histone-lysine N-methyltransferase EHMT2; NG36; lysine N-methyltransferase 1C; KMT1C; HLA-B-associated transcript 8; HLA-B associated transcript 8; G9A; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; Protein G9a; H3-K9-HMTase 3; G9A histone methyltransferase; histone H3-K9 methyltransferase 3; euchromatic histone-lysine N-methyltransferase 2; C6orf30; BAT8; ankyrin repeat-containing protein; GAT8; Histone-lysine N-methyltransferase EHMT2; euchromatic histone lysine methyltransferase 2; anti-G9A/EHMT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-G9A/EHMT2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1160 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human G9A/EHMT2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody AAA211232 (lot AAA211232-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human G9A/EHMT2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody AAA211232 (lot AAA211232-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody AAA211232 (lot AAA211232-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (AAA211232-1) and rabbit anti-G9A/EHMT2 antibody BL4161. For blotting immunoprecipitated G9A/EHMT2, AAA211232 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody AAA211232 (lot AAA211232-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (AAA211232-1) and rabbit anti-G9A/EHMT2 antibody BL4161. For blotting immunoprecipitated G9A/EHMT2, AAA211232 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human G9A/EHMT2 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 (Cat. No. AAA211232 Lot2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human G9A/EHMT2 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 (Cat. No. AAA211232 Lot2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-G9A/EHMT2 antibody
G9A/EHMT2 is a histone H3-Lys-9 methyltransferase. G9A/EHMT2 has been shown to function as a corepressor by associating with transcriptional regulators such as CDP/cut, Blimp-1/PRDI-BF1, and REST/NRSF. G9A/EHMT2 has also been shown to function as a coactivator for a large family of nuclear receptors.
Product Categories/Family for anti-G9A/EHMT2 antibody
NCBI and Uniprot Product Information
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Product Notes
The G9A/EHMT2 ehmt2 (Catalog #AAA211232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-G9A/EHMT2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's G9A/EHMT2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the G9A/EHMT2 ehmt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G9A/EHMT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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