4EBP1 recombinant protein

4EBP1 recombinant protein

Cat. Num. AAA72932

• Gene Names: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
• Applications: Western Blot
• Purity: >75%
• Synonyms: 4EBP1; N/A; 4EBP1 recombinant protein; BP-1; EIF4EBP1; PHAS-I; MGC4316

• Host: E Coli
• Purity/Purification: >75%
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.2 ug/ul (varies by lot)
• Sequence Length: 877

• Applicable Applications for 4EBP1 recombinant protein: WB (Western Blot), Kinase Assay
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human 4EBP1 was expressed in E. coli cells
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for 4EBP1 recombinant protein

Recombinant full-length human 4EBP1 was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: 4EBP1 is a member of a family of translation repressor proteins that directly interact with eukaryotic translation initiation factor 4E (EIF4E). Interaction of 4EBP1 with EIF4E inhibits the multi-subunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs thereby leading to repression of translation. Insulin treatment of adipose cells increases the phosphorylation of 4EBP1 and leads to reduced interaction of 4EBP1 with EIF4E (1). 4EBP1 is expressed in most tissues, with highest levels seen in adipose tissue, pancreas, and skeletal muscle (2).

Product Categories/Family for 4EBP1 recombinant protein

Proteins Substrates; Signaling Reagents - Protein Substrates

References

1. Pause, A. et al: Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5-prime-cap function. Nature371: 762-767, 1994.
2. Tsukiyama-Kohara, K. et al: Tissue distribution, genomic structure, and chromosome mapping of mouse and human eukaryotic initiation factor 4E-binding proteins 1 and 2. Genomics38: 353-363, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 117938308
• NCBI GeneID: 1978
• NCBI Accession #: NP_004086.1
• NCBI GenBank Nucleotide #: NM_004095
• UniProt Accession #: Q13541; Q6IBN3; B2R502; D3DSW8
• Molecular Weight: ~40kDa
• NCBI Official Full Name: Homo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1), mRNA
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E binding protein 1
• NCBI Official Symbol: EIF4EBP1
• NCBI Official Synonym Symbols: BP-1; 4EBP1; 4E-BP1; PHAS-I
• NCBI Protein Information: eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• UniProt Gene Name: EIF4EBP1
• UniProt Synonym Gene Names: 4E-BP1; eIF4E-binding protein 1; PHAS-I
• UniProt Entry Name: 4EBP1_HUMAN