Rabbit anti-Mouse 4EBP1 Polyclonal Antibody | anti-4EBP1 antibody

RABBIT ANTI 4EBP1 (C-TERMINAL)

Cat. Num. AAA49986

• Gene Names: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
• Reactivity: Mouse
• Applications: Western Blot
• Synonyms: 4EBP1, Antibody; RABBIT ANTI 4EBP1 (C-TERMINAL); anti-4EBP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation. ; ; The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble. ; ; This item has been reported as suitable for use in IHC on mouse 3T3 cell cytospins.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 118

• Applicable Applications for anti-4EBP1 antibody: WB (Western Blot)
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); Preparation
• Immunogen: A 14 amino acid peptide located near human 4EBP1 carboxy-terminus.; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Immunocytochemical stainig of 3T3 cells with Rabbit anti Human 4E-BP1 (AAA49986))

Application Data

(Western blot analysis of whole cell lysates from 3t3 mouse embryonic fibroblast cells probed with Rabbit anti Human 4E-BP1 (AAA49986) at 2.5(A), 5(B) and 10(C) ug/ml)

Related Product Information for anti-4EBP1 antibody

MBS221870 recognises an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation. The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble. MBS221870 has been reported as suitable for use in IHC on mouse 3T3 cell cytospins.

NCBI and Uniprot Product Information

• NCBI GI #: 4758258
• NCBI GeneID: 1978
• NCBI Accession #: NP_004086.1
• NCBI GenBank Nucleotide #: NM_004095.3
• UniProt Accession #: Q13541; Q6IBN3; B2R502; D3DSW8
• Molecular Weight: 12,580 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4E-binding protein 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E binding protein 1
• NCBI Official Symbol: EIF4EBP1
• NCBI Official Synonym Symbols: BP-1; 4EBP1; 4E-BP1; PHAS-I
• NCBI Protein Information: eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• UniProt Gene Name: EIF4EBP1
• UniProt Synonym Gene Names: 4E-BP1; eIF4E-binding protein 1; PHAS-I
• UniProt Entry Name: 4EBP1_HUMAN