Human CD105 ELISA Kit | ENG elisa kit

Human CD105 PicoKine ELISA Kit

Cat. Num. AAA45333

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Human
• Synonyms: CD105; N/A; Human CD105 PicoKine ELISA Kit; Endoglin; endoglin; AI528660; AI662476; CD 105; CD105 antigen; Cell surface MJ7/18 antigen; END; ENG; FLJ41744; HHT1; ORW; ORW1; Osler Rendu Weber syndrome 1; OTTMUSP00000012838; OTTMUSP00000012839; RP11 228B15.2; RP23 17P12.4; S endoglin; Transmembrane glycoprotein; ENG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CD105
• Sequence Length: 658
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 15pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human CD105 PicoKine ELISA Kit standard curve)

Related Product Information for ENG elisa kit

Principle of the assay: human CD105 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD105 has been precoated onto 96-well plates. Standards(CHO,E26-G586) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD105 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD105 amount of sample captured in plate.
Background: CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 Theprotein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part ofrecombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 3041681
• NCBI GeneID: 2022
• UniProt Accession #: P17813; Q14248; Q14926; Q5T9C0
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: Endoglin
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin; CD105 antigen
• UniProt Protein Name: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END
• UniProt Entry Name: EGLN_HUMAN