Application Data
(The purity of EZH2 (Y641S) was determined to be >80% by densitometry, approx. MW 130 kDa.)
EZH2 recombinant protein
EZH2 (Y641S) Protein
Gene Names
EZH2; WVS; ENX1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Purity
>80%
Synonyms
EZH2; N/A; EZH2 (Y641S) Protein; EZH2 recombinant protein
Host
Sf9 insect cells using baculovirus
Purity/Purification
>80%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1 ug/ul (varies by lot)
Sequence Length
2655
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
(The purity of EZH2 (Y641S) was determined to be >80% by densitometry, approx. MW 130 kDa.)
Application Data
(The purity of EZH2 (Y641S) was determined to be >80% by densitometry, approx. MW 130 kDa.)
Related Product Information for EZH2 recombinant protein
Description: Recombinant full-length human EZH2 (Y641S) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: EZH2 (enhancer of zeste homolog 2) belongs to the polycomb group (PcG) protein and acts as the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of target genes. EZH2 expression is associated with proliferating tissues and shares an overlapping set of target genes with EZH1. Compared to PRC2-EZH1, PRC2-EZH2 exhibits higher levels of histone methyltransferase (HKMT) activity.
Scientific Background: EZH2 (enhancer of zeste homolog 2) belongs to the polycomb group (PcG) protein and acts as the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of target genes. EZH2 expression is associated with proliferating tissues and shares an overlapping set of target genes with EZH1. Compared to PRC2-EZH1, PRC2-EZH2 exhibits higher levels of histone methyltransferase (HKMT) activity.
Product Categories/Family for EZH2 recombinant protein
References
1. Margueron R, et al: Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms. Mol Cell. 32 (4):503-18, 2008. 2. Shen X, et al: EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency. Mol Cell. 32 (4):491-502, 2008
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
130 kDa
NCBI Official Full Name
Homo sapiens enhancer of zeste homolog 2 (Drosophila), mRNA
NCBI Official Synonym Full Names
enhancer of zeste 2 polycomb repressive complex 2 subunit
NCBI Official Symbol
EZH2
NCBI Official Synonym Symbols
WVS; ENX1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
NCBI Protein Information
histone-lysine N-methyltransferase EZH2
UniProt Protein Name
Histone-lysine N-methyltransferase EZH2
UniProt Gene Name
EZH2
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Product Notes
The EZH2 ezh2 (Catalog #AAA72983) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EZH2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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