Standard Curve (Sample)
General Prothrombin total antigen assay ELISA Kit | F2 elisa kit
Mouse Prothrombin total antigen assay ELISA Kit
Gene Names
F2; Cf2; FII; Cf-2; thrombin
Reactivity
General
Synonyms
Prothrombin total antigen assay; N/A; Mouse Prothrombin total antigen assay ELISA Kit; F2 elisa kit
Reactivity
General
Specificity
This assay recognizes natural mouse prothrombin, thrombin, and thrombin/antithrombin complex. Significant cross reaction is observed with pooled normal plasma from rat. Pooled normal plasma from human, porcine, rabbit, and sheep were assayed for cross-reactivity. No significant cross-reactivity was observed.
Form/Format
Complete Kit
Sequence Length
618
Samples
Mouse plasma, serum, tissue extracts, and cell culture media
Sensitivity
The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.114-0.124) and calculating the corresponding concentration. The MDD was 0.319 ng/ml.
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in ten independent assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C. Shelf Life: 6 months from manufacture, see label for expiration date
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for F2 elisa kit
Intended Uses: This mouse prothrombin/thrombin total antigen assay is intended for the quantitative determination of total prothrombin and thrombin in mouse plasma, serum, tissue extracts, and cell culture media. For quantitation of mouse prothrombin only, refer to the Mouse Prothrombin Antigen Assay (cat # MPTKT). For research use only.
Principle of the Assay: Mouse prothrombin, thrombin, and thrombin-antithrombin complex will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prothrombin. The amount of color development is directly proportional to the concentration of prothrombin in the sample.
Background: Prothrombin (aka Factor II) is a single-chain vitamin K dependent 579 amino acid glycoprotein zymogen [1]. Prothrombin is proteolytically activated to thrombin by the prothrombinase enzyme complex in the coagulation cascade common pathway. The serine protease thrombin converts plasma fibrinogen to insoluble fibrin. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease [2]. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210 [3].
Principle of the Assay: Mouse prothrombin, thrombin, and thrombin-antithrombin complex will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prothrombin. The amount of color development is directly proportional to the concentration of prothrombin in the sample.
Background: Prothrombin (aka Factor II) is a single-chain vitamin K dependent 579 amino acid glycoprotein zymogen [1]. Prothrombin is proteolytically activated to thrombin by the prothrombinase enzyme complex in the coagulation cascade common pathway. The serine protease thrombin converts plasma fibrinogen to insoluble fibrin. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease [2]. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210 [3].
Product Categories/Family for F2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70,269 Da
NCBI Official Full Name
prothrombin
NCBI Official Synonym Full Names
coagulation factor II
NCBI Official Symbol
F2
NCBI Official Synonym Symbols
Cf2; FII; Cf-2; thrombin
NCBI Protein Information
prothrombin
UniProt Protein Name
Prothrombin
UniProt Gene Name
F2
UniProt Synonym Gene Names
Cf2
UniProt Entry Name
THRB_MOUSE
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Product Notes
The General F2 f2 (Catalog #AAA37749) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA37749 ELISA Kit recognizes General F2. It is sometimes possible for the material contained within the vial of "Prothrombin total antigen assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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