Standard Curve (Sample)
General Prekallikrein total antigen assay ELISA Kit | KLK1 elisa kit
Human Prekallikrein total antigen assay ELISA Kit
Gene Names
KLK1; hK1; KLKR; Klk6
Reactivity
General
Synonyms
Prekallikrein total antigen assay; N/A; Human Prekallikrein total antigen assay ELISA Kit; KLK1 elisa kit
Reactivity
General
Form/Format
Complete Kit
Sequence Length
262
Samples
Human plasma
Preparation and Storage
Store at 4 degree C. Shelf Life: 6 months from manufacture, see label for expiration date
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for KLK1 elisa kit
Intended Uses: This human prekallikrein total antigen assay is intended for the quantitative determination of total prekallikrein antigen in human plasma.
Principle of the Assay: Human prekallikrein will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, affinity purified polyclonal anti-human prekallikrein primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human prekallikrein. Color development is proportional to the total concentration of prekallikrein in the samples.
Background: Prekallikrein is the glycosylated single chain zymogen precursor of the plasma serine protease kallikrein. Plasma prekallikrein circulates with kininogen and is activated by Factor XIIa in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time [1]. Elevated plasma prekallikrein is associated with diabetes [2] and cardiovascular disease [3].
Principle of the Assay: Human prekallikrein will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, affinity purified polyclonal anti-human prekallikrein primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human prekallikrein. Color development is proportional to the total concentration of prekallikrein in the samples.
Background: Prekallikrein is the glycosylated single chain zymogen precursor of the plasma serine protease kallikrein. Plasma prekallikrein circulates with kininogen and is activated by Factor XIIa in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time [1]. Elevated plasma prekallikrein is associated with diabetes [2] and cardiovascular disease [3].
Product Categories/Family for KLK1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,890 Da
NCBI Official Full Name
kallikrein-1 preproprotein
NCBI Official Synonym Full Names
kallikrein 1
NCBI Official Symbol
KLK1
NCBI Official Synonym Symbols
hK1; KLKR; Klk6
NCBI Protein Information
kallikrein-1; tissue kallikrein; glandular kallikrein 1; kallikrein serine protease 1; kallikrein 1, renal/pancreas/salivary; kidney/pancreas/salivary gland kallikrein
UniProt Protein Name
Kallikrein-1
UniProt Gene Name
KLK1
UniProt Entry Name
KLK1_HUMAN
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Product Notes
The General KLK1 klk1 (Catalog #AAA37762) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA37762 ELISA Kit recognizes General KLK1. It is sometimes possible for the material contained within the vial of "Prekallikrein total antigen assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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