CD2 active protein

CD2 Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA257956

• Gene Names: CD2; T11; SRBC; LFA-2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD2; N/A; CD2 Protein, Human, Recombinant (ECD, hFc Tag); Human CD2 Protein (Fc Tag); LFA-2 Protein, Human; SRBC Protein, Human; T11 Protein, Human; CD2 molecule; CD2 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp209

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Lys 25
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CD2 (NP_001758.2)(Met1-Asp209) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD58-His at 10ug/mL (100uL/well) can bind human CD2-Fc. The EC50 of human CD2-Fc is 100-350ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized human CD58-His at 10 ug/mL (100 uL/well) can bind human CD2-Fc, the EC50 of human CD2-Fc is 100-350 ng/mL.)

SDS-PAGE

Related Product Information for CD2 active protein

Background: T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, and SRBC, is a single-pass type I membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). CD2 has been shown to set quantitative thresholds in T cell activation both in vivo and in vitro. Further, intracellular CD2 signaling pathways and networks are being discovered by the identification of several cytosolic tail binding proteins. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. As a cell surface glycoprotein, CD2 expressed on most human T cells and natural killer (NK) cells and plays an important role in mediating cell adhesion in both T-lymphocytes and in signal transduction.

References

Yang JJ, et al. (2001) Structural biology of the cell adhesion protein CD2: alternatively folded states and structure-function relation. Curr Protein Pept Sci. 2(1): 1-17.
Wilkins AL, et al. (2003) Structural biology of the cell adhesion protein CD2: from molecular recognition to protein folding and design. Curr Protein Pept Sci. 4(5): 367-73.
McNerney ME, et al. (2006) The CD2 family of natural killer cell receptors. Curr Top Microbiol Immunol. 298: 91-120.

NCBI and Uniprot Product Information

• NCBI GI #: 156071472
• NCBI GeneID: 914
• NCBI Accession #: NP_001758.2
• NCBI GenBank Nucleotide #: NM_001767.3
• UniProt Accession #: P06729; Q96TE5
• Molecular Weight: 39,448 Da
• NCBI Official Full Name: T-cell surface antigen CD2
• NCBI Official Synonym Full Names: CD2 molecule
• NCBI Official Symbol: CD2
• NCBI Official Synonym Symbols: T11; SRBC; LFA-2
• NCBI Protein Information: T-cell surface antigen CD2; CD2 antigen (p50), sheep red blood cell receptor; LFA-3 receptor; T-cell surface antigen T11/Leu-5; erythrocyte receptor; lymphocyte-function antigen-2; rosette receptor
• UniProt Protein Name: T-cell surface antigen CD2
• UniProt Gene Name: CD2
• UniProt Synonym Gene Names: SRBC
• UniProt Entry Name: CD2_HUMAN