anti-Human CD43 Antibody | anti-CD43 antibody

Anti-Hu CD43 APC

Cat. Num. AAA129246

• Gene Names: SPN; LSN; CD43; GALGP; GPL115
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD43; N/A; Anti-Hu CD43 APC; sialophorin; Leukosialin; Sialophorin; Galactoglycoprotein; GALGP; LSN; SPN; GP5; anti-CD43 antibody

• Reactivity: Human
• Isotype: Mouse IgG1
• Clone Number: MEM-59
• Specificity: The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
• Form/Format: APC; Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m

• Applicable Applications for anti-CD43 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Human T lymphocytes.
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD43 positive lymphocytes (red-filled) from CD43 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD43 (MEM-59) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD43 (MEM-59) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD43 antibody

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.

References

*Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11, URL: http://www.ncbi.nlm.nih.gov/pubmed/26396269,*Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/17098248,*Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/11773067?dopt=,*Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/18165900?ordin,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL:,*Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE: MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol. 1995 Apr;25(4):1051-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/7537669?dopt=A,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL:,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL:,*Stefanova I, Hilgert I, Angelisova P, Kristofova H, Horejsi V: Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia Biol (Praha). 1988;34(4):255-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/2467832?dopt=A,
*Kadaja L, Laos S, Maimets T: Overexpression of leukocyte marker CD43 causes activation of the tumor suppressor proteins p53 and ARF. Oncogene. 2004 Apr 1;23(14):2523-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/14676827?ordin,*Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermák L, And?ra L, Ho?ejší V, Stockinger H: LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147. Mol Immunol. 2007 Nov 8, URL: https://www.ncbi.nlm.nih.gov/pubmed/17996943?ordin,*Kadaja-Saarepuu L, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T: CD43 promotes cell growth and helps to evade FAS-mediated apoptosis in non-hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. Oncogene. 2007 Sep 24, URL: https://www.ncbi.nlm.nih.gov/pubmed/17891181?ordin,*Mody PD, Cannon JL, Bandukwala HS, Blaine KM, Schilling AB, Swier K, Sperling AI: Signaling through CD43 regulates CD4 T-cell trafficking. Blood. 2007 Oct 15;110(8):2974-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/17638845?ordin,*Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol. 1989 nFeb;26(2):153-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/2918859?dopt=A,

NCBI and Uniprot Product Information

• NCBI GI #: 71979937
• NCBI GeneID: 6693
• NCBI Accession #: NP_001025459.1
• NCBI GenBank Nucleotide #: NM_001030288.2
• UniProt Accession #: P16150; A8K9B1
• Molecular Weight: 40,322 Da
• NCBI Official Full Name: leukosialin
• NCBI Official Synonym Full Names: sialophorin
• NCBI Official Symbol: SPN
• NCBI Official Synonym Symbols: LSN; CD43; GALGP; GPL115
• NCBI Protein Information: leukosialin; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43)
• UniProt Protein Name: Leukosialin
• UniProt Gene Name: SPN
• UniProt Synonym Gene Names: CD43; GALGP
• UniProt Entry Name: LEUK_HUMAN