IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-p40 antibody 14D5E8F12 . Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14D5E8F12 (AAA72367) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72367, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human p40 Antibody | anti-p40 antibody
Anti-p40 [14D5E8F12]
Gene Names
TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
Purified. Protein A affinity purified
Synonyms
p40; N/A; Anti-p40 [14D5E8F12]; TP63; CUSP; Tumor protein 63; chronic ulcerative stomatitis protein; Keratinocyte transcription factor KET; Transformation-related protein 63; Tumor protein p73-like; p73L; p51; ZR8; anti-p40 antibody
Reactivity
Human
Isotype
Rabbit IgG kappa
Clone Number
14D5E8F12
Specificity
This antibody is specific for the p40 peptide of the human p63. P63 protein acts as a sequence specific DNA binding transcriptional activator or repressor. It is also involved in notch signaling by probably inducing JAG1 and JAG2 and plays a role in the regulation of epithelial morphogenesis. The p63 molecule has a high degree of structural homology with p53 and has three similar functional domains: N-terminal transcription activation region, core DNA binding region, and C-terminal oligomerization region.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-p40 antibody
IHC (Immunohistochemistry), ELISA
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-p40 antibody 14D5E8F12 . Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14D5E8F12 (AAA72367) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72367, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-p40 antibody 14D5E8F12 . Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14D5E8F12 (AAA72367) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72367, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-p40 antibody 14D5E8F12 . Paraformaldehyde fixed A431 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 14D5E8F12 (AAA72367, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-p40 antibody 14D5E8F12 . Paraformaldehyde fixed A431 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 14D5E8F12 (AAA72367, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-p40 antibody
The original antibody was generated by immunizing mice with p40 peptide comprising amino acids 4-19 (LENNAQTQFSEPQYTN) of the p63 protein (Uniprot -Q9H3D4). The p40 peptide had a sulfhydryl group at its N-terminus and a carrier protein KLH to improve its immunogenicity.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Product Categories/Family for anti-p40 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
76,785 Da
NCBI Official Full Name
tumor protein 63 isoform 2
NCBI Official Synonym Full Names
tumor protein p63
NCBI Official Symbol
TP63
NCBI Official Synonym Symbols
AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
NCBI Protein Information
tumor protein 63; CUSP; transformation-related protein 63; tumor protein p53-competing protein; amplified in squamous cell carcinoma; chronic ulcerative stomatitis protein; keratinocyte transcription factor KET; tumor protein p63 deltaN isoform delta
UniProt Protein Name
Tumor protein 63
UniProt Gene Name
TP63
UniProt Synonym Gene Names
KET; P63; P73H; P73L; TP73L; p63; CUSP; TP63; p73L
UniProt Entry Name
P63_HUMAN
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Product Notes
The p40 tp63 (Catalog #AAA72367) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-p40 [14D5E8F12] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p40 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the p40 tp63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p40, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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