WB (Western Blot)
(Detection of human p40 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, RT-4, A2058, A-431, and BJ cells prepared using NETN lysis buffer. Antibody: Mouse anti-p40 monoclonal antibody [BC28] (AAA213508 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
Mouse anti-Human p40 Monoclonal Antibody | anti-p40 antibody
Mouse anti-p40 Monoclonal Antibody [BC28]
Gene Names
TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
p40, Antibody; Mouse anti-p40 Monoclonal Antibody [BC28]; p63; p73H; p73L; RHS; SHFM4; Tumor protein p73-like; TP53L; transformation-related protein 63; tumor protein 63; tumor protein p53-competing protein; p53CP; TP53CP; TP73L; CUSP; p51; TP63; AIS; amplified in squamous cell carcinoma; B(p51A); chronic ulcerative stomatitis protein; EEC3; keratinocyte transcription factor KET; KET; LMS; NBP; OFC8; p40; B(p51B); Tumor protein 63; tumor protein p63; anti-p40 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
BC28
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for anti-p40 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human p40 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, RT-4, A2058, A-431, and BJ cells prepared using NETN lysis buffer. Antibody: Mouse anti-p40 monoclonal antibody [BC28] (AAA213508 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human p40 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, RT-4, A2058, A-431, and BJ cells prepared using NETN lysis buffer. Antibody: Mouse anti-p40 monoclonal antibody [BC28] (AAA213508 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IHC (Immunohistochemistry)
(Detection of human p40 in lung carcinoma by IHC. Antibody: Mouse anti-p40 monoclonal antibody [BC28] (AAA213508). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB)
IHC (Immunohistochemistry)
(Detection of human p40 in lung carcinoma by IHC. Antibody: Mouse anti-p40 monoclonal antibody [BC28] (AAA213508). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB)
Related Product Information for anti-p40 antibody
Tumor protein p63 is a member of the p53 family of transcription factors. The functional domains of p53 family proteins include an N-terminal transactivation domain, a central DNA-binding domain and an oligomerization domain. Alternative splicing of this gene and the use of alternative promoters results in multiple transcript variants encoding different isoforms that vary in their functional properties. These isoforms function during skin development and maintenance, adult stem/progenitor cell regulation, heart development and premature aging. P63 is an extremely sensitive marker of lung squamous cell carcinoma, but it suffers from low specificity due to its reactivity in a substantial proportion of lung adenocarcinomas and other tumor types. P40 is a splice isoform of p63 known as DeltaNp63 and has been demonstrated to be highly specific for squamous/basal cells making it is markedly superior to p63 in specificity [adapted from NCBI Entrez Gene (Gene ID: 8626)].
Product Categories/Family for anti-p40 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65,288 Da
NCBI Official Full Name
tumor protein 63 isoform 1
NCBI Official Synonym Full Names
tumor protein p63
NCBI Official Symbol
TP63
NCBI Official Synonym Symbols
AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
NCBI Protein Information
tumor protein 63
UniProt Protein Name
Tumor protein 63
UniProt Gene Name
TP63
UniProt Synonym Gene Names
KET; P63; P73H; P73L; TP73L; p63; CUSP; TP63; p73L
UniProt Entry Name
P63_HUMAN
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Product Notes
The p40 tp63 (Catalog #AAA213508) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-p40 Monoclonal Antibody [BC28] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p40 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the p40 tp63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p40, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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