PD-1 Antibody | anti-PD-1 antibody

Anti-PD-1 [5C4.B8 (Nivolumab)]

Cat. Num. AAA72300

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Human, Cynomolgus
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified. Protein A affinity purified
• Synonyms: PD-1; N/A; Anti-PD-1 [5C4.B8 (Nivolumab)]; CD279; programmed death-1; programmed death 1 ; PD1; PD 1; Programmed cell death protein 1; hPD-1; hPD1; hPD 1; BMS-936558; MDX-1106;ONO-4538; anti-PD-1 antibody

• Reactivity: Human, Cynomolgus
• Isotype: Rabbit IgG kappa
• Clone Number: 5C4.B8 (Nivolumab)
• Specificity: Nivolumab binds to the extracellular portion of human PD-1 (2.6 nM - Scatchard analysis and SPR) - the antibody also binds to cynomolgus PD-1 with a similar affinity (3.9 nM - SPR). The antibody does not bind to other immunoglobulin superfamily proteins such as CD28, CTLA-4, ICOS and BTLA. The epitope of Nivolumab for both human and cynomolgus PD-1 includes the sequences SFVLNWYR-MSPSNQTDKLAAFPEDR (aa 29-53) and SGTYLCGAISLAPKAQIKE (aa 85-103), as shown by mass spectrometry of protease-treated fragments of PD-1 - these residues are thought to additionally be important for liand binding to PD-1. PD-1 is an inhibitory receptor expressed on the surface of T cells. It is able to bind to its ligands PDL-1 and PDL-2 which results in an inhibitory signal leading to decreased T cell proliferation, cytokine protuction and cytotoxic activtiy. PDL-1 is often expressed in human tumors such as melanoma, lung and kidney where it is able to overactivate PD-1 and plays a role in the evasion of cancer cells from the immune system.
• Purity/Purification: Purified. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PD-1 antibody: Blocking, SPR (Surface Plasmon Resonance), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow-cytometry using anti-PD-1 antibody Nivolumab Cynomolgus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Nivolumab (AAA72300, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.)

FCM/FACS (Flow Cytometry)

(Flow-cytometry using anti-PD-1 antibody Nivolumab Rhesus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Nivolumab (AAA72300, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.)

Related Product Information for anti-PD-1 antibody

mAb PD1.5 was prepared by immunizing IgH and IgK knock-out transgenic mice possessing a human immunoglobulin (heavy chain) minilocus with recombinant human PD-1-Fc protein consisting of the extracellular domain of PD-1 (amino acids 1-167) and the Fc portion of human IgG1, and Chinese hamster ovary (CHO) cells expressing human PD-1. Nivolumab was generated by grafting the variable regions of PD1.5 onto human kappa and IgG4 constant regions containing an S228P mutation (prevents Fab arm exchange with endogenous IgG4 antibodies).
Describes the generation and characterization of the anti-PD-1 antibody nivolumab, including its specicity, binding affinity and its in vivo and in vitro properties.
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.

Product Categories/Family for anti-PD-1 antibody

cancer; melanoma; lung cancer; kidney cancer; oncology; immune system; adaptive immunity; cytokines; biosimilar

References

Wang et al.
Cancer Immunol Res. 2014 Sep;2(9):846-56.

NCBI and Uniprot Product Information

• NCBI GI #: 145559515
• NCBI GeneID: 5133
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: Programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1