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FCM/FACS (Flow Cytometry)
(Figure 4. Flow cytometry analysis with 1ug/mL Canine PD-1 Protein, hFc Tag(AAA129506) on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
PD-1 recombinant protein
Canine PD-1 Protein, hFc Tag
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
PD-1; N/A; Canine PD-1 Protein, hFc Tag; CD279; PD1; SLEB2; hPD-1; hPD-l; hSLE1; Recombinant Canine PD-1 protein with C-terminal human Fc tag; PD-1 recombinant protein
Host
HEK293
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Sequence
PD-1 (Leu25-Gly168) hFc (Glu99-Ala330)
Tag
C-Human Fc
Preparation and Storage
Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
FCM/FACS (Flow Cytometry)
(Figure 4. Flow cytometry analysis with 1ug/mL Canine PD-1 Protein, hFc Tag(AAA129506) on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
FCM/FACS (Flow Cytometry)
(Figure 4. Flow cytometry analysis with 1ug/mL Canine PD-1 Protein, hFc Tag(AAA129506) on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
ELISA
(Figure 3. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Canine PD-1 Protein, hFc Tag (AAA129506) can bind Canine PD-L1 Protein, mFc Tag in a linear range of 16–400 ng/mL.)
ELISA
(Figure 3. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Canine PD-1 Protein, hFc Tag (AAA129506) can bind Canine PD-L1 Protein, mFc Tag in a linear range of 16–400 ng/mL.)
ELISA
(Figure 2. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Canine PD-1 Protein, hFc Tag (AAA129506) can bind Anti-Canine PD-1(INTERVET 4F12) mAb in a linear range of 0.64–80 ng/mL.)
ELISA
(Figure 2. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Canine PD-1 Protein, hFc Tag (AAA129506) can bind Anti-Canine PD-1(INTERVET 4F12) mAb in a linear range of 0.64–80 ng/mL.)
SDS-PAGE
(Figure 1. Canine PD-1 Protein, hFc Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Canine PD-1 Protein, hFc Tag on SDS-PAGE under reducing condition.)
Related Product Information for PD-1 recombinant protein
Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020]
Product Categories/Family for PD-1 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
The protein has a predicted molecular mass of 42.3kDa after removal of the signal peptide. The apparent molecular mass of dPD-1-hFc is approximately 55-70kDa due to glycosylation.
Similar Products
Product Notes
The PD-1 (Catalog #AAA129506) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: PD-1 (Leu25-Gly 168) hFc (Glu99-Ala 330). It is sometimes possible for the material contained within the vial of "PD-1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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