PD-1 recombinant antibody

PD-1 Single Domain Antibody [F25F4]

Cat. Num. AAA40973

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Purity: PD-1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Synonyms: PD-1, Recombinant Antibody; PD-1 Single Domain Antibody [F25F4]; PD-1 Antibody: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; PD1; Programmed cell death protein 1; Protein PD-1; PDCD1; PDCD-1; programmed cell death 1; PD-1 recombinant antibody

• Host: Llama
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: F2-5F4
• Purity/Purification: PD-1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Form/Format: Liquid; PD-1 Antibody is supplied in PBS.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for PD-1 recombinant antibody: FCM/FACS (Flow Cytometry), ELISA
• Immunogen: PD-1 antibody was raised against a recombinant protein corresponding to amino acids 25 - 167 of human PD-1.
• Research Area: Immunology, Cancer, Cell Cycle
• NCBI Organism: Homo sapiens
• Preparation and Storage: PD-1 antibody should be stored in working aliquots at -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

FCM/FACS (Flow Cytometry)

(Flow cytometry analysis of PD-1 transfected 293 cells using PD-1 [F2-5F4] antibody at 10ug/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.)

ELISA

(Titration ELISA of PD-1.)

Related Product Information for PD-1 recombinant antibody

PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

Product Categories/Family for PD-1 recombinant antibody

Primary Antibodies

References

Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.

NCBI and Uniprot Product Information

• NCBI GI #: 145559515
• NCBI GeneID: 5133
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: Predicted: 32 kDa; Observed: 38 kDa
• NCBI Official Full Name: Programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1