FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-PD-1antibody29F.1A12(AAA72538). Mousesplenocyteswerestainedwithanisotypecontrolortherabbit-chimericversionof29F.1A12(AAA72538,blueline)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusingAlexaFluor488conjugatedgoatanti-rabbitIgGandcellsanalysedonaFACSCantoflow-cytometer.)
PD-1 recombinant antibody
Anti-PD-1 [29F.1A12], Rabbit IgG, kappa
Gene Names
Pdcd1; PD-1; Pdc1; Ly101
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
PD-1, Recombinant Antibody; Anti-PD-1 [29F.1A12], Rabbit IgG, kappa; PD-1; Anti-PD-1 [29F.1A12]; CD279; Programmed cell death protein 1; Protein PD-1; mPD-1; PD-1 recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
29F.1A12
Specificity
This antibody recognizes an epitope on mouse PD-1.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for PD-1 recombinant antibody
IHC (Immunohistochemistry), Blocking, WB (Western Blot), FCM/FACS (Flow Cytometry)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-PD-1antibody29F.1A12(AAA72538). Mousesplenocyteswerestainedwithanisotypecontrolortherabbit-chimericversionof29F.1A12(AAA72538,blueline)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusingAlexaFluor488conjugatedgoatanti-rabbitIgGandcellsanalysedonaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-PD-1antibody29F.1A12(AAA72538). Mousesplenocyteswerestainedwithanisotypecontrolortherabbit-chimericversionof29F.1A12(AAA72538,blueline)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusingAlexaFluor488conjugatedgoatanti-rabbitIgGandcellsanalysedonaFACSCantoflow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescencestainingofmousesplenocyteswithanti-PD-1(AAA72538)29F.1A12 ImmunofluorescenceanalysisofparaformaldehydefixedmousesplenocytesstainedwiththechimericrabbitIgGversionof29F.1A12(AAA72538)(1:1000dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1500dilution),showingnuclearstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72538,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasanti-fluoresceinantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingofmousesplenocyteswithanti-PD-1(AAA72538)29F.1A12 ImmunofluorescenceanalysisofparaformaldehydefixedmousesplenocytesstainedwiththechimericrabbitIgGversionof29F.1A12(AAA72538)(1:1000dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1500dilution),showingnuclearstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72538,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasanti-fluoresceinantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot)
(Western Blotusinganti-PD-1antibody29F.1A12(AAA72538). Mousethymus(1ug/ml)lysate(35ugproteininRIPAbuffer)wasresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofX77(AAA72538)beforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-PD-1antibody29F.1A12(AAA72538). Mousethymus(1ug/ml)lysate(35ugproteininRIPAbuffer)wasresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofX77(AAA72538)beforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
Related Product Information for PD-1 recombinant antibody
The antibody was raised by immunizing rats with plasmid DNA containing PD-1.
References
Liang et al. Regulation of PD-1, PD-L1, and PD-L2 expressionduring normal and autoimmune response Eur. J. Immunol. 2003. 33: 2706-2716 N/A14515254
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
31,842 Da
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
Pdcd1
NCBI Official Synonym Symbols
PD-1; Pdc1; Ly101
NCBI Protein Information
programmed cell death protein 1; mPD-1; protein PD-1
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
Pdcd1
UniProt Synonym Gene Names
Pd1; Protein PD-1; mPD-1
UniProt Entry Name
PDCD1_MOUSE
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Product Notes
The PD-1 pdcd1 (Catalog #AAA72538) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PD-1 [29F.1A12], Rabbit IgG, kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PD-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), Blocking, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PD-1 pdcd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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