Human 28S ribosomal protein S21, mitochondrial (MRPS21) ELISA Kit | MRPS21 elisa kit

Human 28S ribosomal protein S21, mitochondrial (MRPS21) ELISA Kit

Cat. Num. AAA245865

• Gene Names: MRPS21; MDS016; RPMS21; MRP-S21
• Reactivity: Human
• Synonyms: 28S ribosomal protein S21, mitochondrial (MRPS21); N/A; Human 28S ribosomal protein S21, mitochondrial (MRPS21) ELISA Kit; MRPS21 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MRPS21. No significant cross-reactivity or interference between MRPS21 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MRPS21 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for MRPS21 elisa kit

Principle of the Assay: MRPS21 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MRPS21 antibody and an MRPS21-HRP conjugate. The assay sample and buffer are incubated together with MRPS21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MRPS21 concentration since MRPS21 from samples and MRPS21-HRP conjugate compete for the anti-MRPS21 antibody binding site. Since the number of sites is limited, as more sites are occupied by MRPS21 from the sample, fewer sites are left to bind MRPS21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MRPS21 concentration in each sample is interpolated from this standard curve.

Intended Uses: This MRPS21 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MRPS21. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 16950593
• NCBI GeneID: 54460
• NCBI Accession #: NP_061870.1
• NCBI GenBank Nucleotide #: NM_018997.3
• Molecular Weight: 10,742 Da
• NCBI Official Full Name: 28S ribosomal protein S21, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S21
• NCBI Official Symbol: MRPS21
• NCBI Official Synonym Symbols: MDS016; RPMS21; MRP-S21
• NCBI Protein Information: 28S ribosomal protein S21, mitochondrial; S21mt; mitochondrial 28S ribosomal protein S21
• UniProt Protein Name: 28S ribosomal protein S21, mitochondrial
• UniProt Gene Name: MRPS21
• UniProt Synonym Gene Names: RPMS21; MDS016; MRP-S21; S21mt
• UniProt Entry Name: RT21_HUMAN