Standard Curve (Sample)
(Fig.1. Human 28S ribosomal protein S21, mitochondrial (MRPS21) Standard Curve.)
Human 28S Ribosomal Protein S21, Mitochondrial (MRPS21) ELISA Kit | MRPS21 elisa kit
Human 28S Ribosomal Protein S21, Mitochondrial (MRPS21) ELISA Kit
Gene Names
MRPS21; MDS016; RPMS21; MRP-S21
Reactivity
Human
Synonyms
28S Ribosomal Protein S21, Mitochondrial (MRPS21); N/A; Human 28S Ribosomal Protein S21, Mitochondrial (MRPS21) ELISA Kit; MRPS21; MDS016; MRP-S21; RPMS21; mitochondrial 28S ribosomal protein S21; MRPS21 elisa kit
Reactivity
Human
Specificity
Human 28S ribosomal protein S21, mitochondrial (MRPS21) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRPS21. No significant cross-reactivity or interference between Human MRPS21 and analogues was observed.
Sequence Length
87
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human 28S ribosomal protein S21, mitochondrial (MRPS21) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human 28S ribosomal protein S21, mitochondrial (MRPS21) Standard Curve.)
Related Product Information for MRPS21 elisa kit
Description: This Human 28S ribosomal protein S21, mitochondrial (MRPS21) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS21 in samples. An antibody specific for MRPS21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MRPS21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS21 encodes a 28S subunit protein, mitochondrial ribosomal protein S21 that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to MRPS21 are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein.
Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS21 encodes a 28S subunit protein, mitochondrial ribosomal protein S21 that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to MRPS21 are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
28S ribosomal protein S21, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S21
NCBI Official Symbol
MRPS21
NCBI Official Synonym Symbols
MDS016; RPMS21; MRP-S21
NCBI Protein Information
28S ribosomal protein S21, mitochondrial
UniProt Protein Name
28S ribosomal protein S21, mitochondrial
UniProt Gene Name
MRPS21
UniProt Synonym Gene Names
RPMS21; MRP-S21; S21mt
UniProt Entry Name
RT21_HUMAN
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Product Notes
The Human MRPS21 mrps21 (Catalog #AAA331692) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331692 ELISA Kit recognizes Human MRPS21. It is sometimes possible for the material contained within the vial of "28S Ribosomal Protein S21, Mitochondrial (MRPS21), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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