Standard Curve (Sample)
(Fig.1. Human Alpha-Actinin 1 (ACTN-1) Standard Curve.)
Human Alpha-Actinin 1 (ACTN-1) ELISA Kit | ACTN-1 elisa kit
Human Alpha-Actinin 1 (ACTN-1) ELISA Kit
Gene Names
ACTN1; BDPLT15
Reactivity
Human
Synonyms
Alpha-Actinin 1 (ACTN-1); N/A; Human Alpha-Actinin 1 (ACTN-1) ELISA Kit; ACTN1; FLJ40884; FLJ54432; F-actin cross-linking protein; actinin 1 smooth muscle; alpha-actinin 1; ACTN-1 elisa kit
Reactivity
Human
Specificity
Human Alpha-Actinin 1 (ACTN-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACTN1. No significant cross-reactivity or interference between Human ACTN1 and analogues was observed.
Sequence Length
892
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Alpha-Actinin 1 (ACTN-1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Alpha-Actinin 1 (ACTN-1) Standard Curve.)
Related Product Information for ACTN-1 elisa kit
Description: This Human Alpha-Actinin 1 (ACTN-1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTN1 in samples. An antibody specific for ACTN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha-actinin-1 is a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.
Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha-actinin-1 is a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-actinin-1 isoform b
NCBI Official Synonym Full Names
actinin alpha 1
NCBI Official Symbol
ACTN1
NCBI Official Synonym Symbols
BDPLT15
NCBI Protein Information
alpha-actinin-1
UniProt Protein Name
Alpha-actinin-1
UniProt Gene Name
ACTN1
UniProt Entry Name
ACTN1_HUMAN
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Product Notes
The Human ACTN-1 actn1 (Catalog #AAA331088) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331088 ELISA Kit recognizes Human ACTN-1. It is sometimes possible for the material contained within the vial of "Alpha-Actinin 1 (ACTN-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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