Human Amidophosphoribosyltransferase (PPAT) ELISA Kit | PPAT elisa kit

Human Amidophosphoribosyltransferase (PPAT) ELISA Kit

Cat. Num. AAA244376

• Gene Names: Ppat; Atase
• Reactivity: Human
• Synonyms: Amidophosphoribosyltransferase (PPAT); N/A; Human Amidophosphoribosyltransferase (PPAT) ELISA Kit; PPAT elisa kit

• Reactivity: Human
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/ml
• Sensitivity: 1.0ng/mL

• Intended Uses: This PPAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PPAT. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PPAT elisa kit

Principle of the assay: PPAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PPAT antibody and an PPAT-HRP conjugate. The assay sample and buffer are incubated together with PPAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPAT concentration since PPAT from samples and PPAT-HRP conjugate compete for the anti-PPAT antibody binding site. Since the number of sites is limited, as more sites are occupied by PPAT from the sample, fewer sites are left to bind PPAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPAT concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 17105352
• NCBI GeneID: 117544
• NCBI Accession #: NP_476546.1
• NCBI GenBank Nucleotide #: NM_057198.2
• Molecular Weight: 57,437 Da
• NCBI Official Full Name: amidophosphoribosyltransferase
• NCBI Official Synonym Full Names: phosphoribosyl pyrophosphate amidotransferase
• NCBI Official Symbol: Ppat
• NCBI Official Synonym Symbols: Atase
• NCBI Protein Information: amidophosphoribosyltransferase; GPAT; glutamine phosphoribosylpyrophosphate amidotransferase
• UniProt Protein Name: Amidophosphoribosyltransferase
• UniProt Gene Name: Ppat
• UniProt Synonym Gene Names: ATase; GPAT
• UniProt Entry Name: PUR1_RAT