Standard Curve (Sample)
Human CCL3 ELISA Kit | CCL3 elisa kit
Human CCL3 ELISA Kit
Gene Names
CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
Reactivity
Human
Applications
ELISA
Synonyms
CCL3; N/A; Human CCL3 ELISA Kit; G0S19-1; MIP1A; SCYA3; C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein; CCL3 elisa kit
Reactivity
Human
Specificity
The Human CCL3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or
Sequence Length
92
Samples
Sandwich Enzyme-Linked Immunosorbent Assay for Quantitative Detection of Human CCL3 Concentrations in Cell Culture Supernatants, Serum, Plasma
Sensitivity
10 pg/ml
Applicable Applications for CCL3 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CCL3 elisa kit
Principle of the Assay: The Human CCL3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL3 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CCL3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CCL3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
Background/Introduction: Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,085 Da
NCBI Official Full Name
C-C motif chemokine 3
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
CCL3
NCBI Official Synonym Symbols
MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
NCBI Protein Information
C-C motif chemokine 3; SIS-beta; PAT 464.1; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a)
UniProt Protein Name
C-C motif chemokine 3
UniProt Gene Name
CCL3
UniProt Synonym Gene Names
G0S19-1; MIP1A; SCYA3; MIP-1-alpha
UniProt Entry Name
CCL3_HUMAN
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Product Notes
The Human CCL3 ccl3 (Catalog #AAA105561) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA105561 ELISA Kit recognizes Human CCL3. AAA Biotech's CCL3 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the CCL3 ccl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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