Reactivity
Human
Specificity
Human CCL3 ELISA Kit has high sensitivity and excellent specificity for detection of HumanCCL3. No significant cross-reactivity or interference between Human CCL3 and analogues was observed.
Assay Type
Sandwich
Samples
Serum, Plasma, Cell culture supernatants
Detection Range
31.25 pg/mL-2,000 pg/mL
Sensitivity
16 pg/mL
Applicable Applications for CCL3 elisa kit
ELISA
Intra-assay Precision
Intra-assay Precision: The CV (%)?10%.
Inter-assay Precision
Inter-assay Precision :The CV (%)?12%
Preparation and Storage
The unopened kit should be stored at 2 - 8 degree C. After opening, please store refer to protocols.
Related Product Information for CCL3 elisa kit
Principle of the Assay: Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a maturepeptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis.Human CCL3 ELISA Kitemploys a double antibody sandwich method to quantitate Human CCL3 in samples. An antibody specific for Human CCL3 hasbeen pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human CCL3 present is bound bythe immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Human CCL3 isadded to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove anyunbound streptavidin-enzyme reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellowafter adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human CCL3concentration is proportional to the OD450 nm value.
Product Categories/Family for CCL3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
10,085 Da
NCBI Official Full Name
macrophage inflammatory protein 1 alpha
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
CCL3
NCBI Official Synonym Symbols
MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
NCBI Protein Information
C-C motif chemokine 3; SIS-beta; PAT 464.1; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a)
UniProt Protein Name
C-C motif chemokine 3
UniProt Gene Name
CCL3
UniProt Synonym Gene Names
G0S19-1; MIP1A; SCYA3; MIP-1-alpha
UniProt Entry Name
CCL3_HUMAN
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Product Notes
The Human CCL3 ccl3 (Catalog #AAA332531) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332531 ELISA Kit recognizes Human CCL3. AAA Biotech's CCL3 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CCL3 ccl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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