Standard Curve (Sample)
(Fig.1. Human Cytochrome b-c1 complex subunit 10 (UQCR) Standard Curve.)
Human Cytochrome B-c1 Complex Subunit 10 (UQCR) ELISA Kit | UQCR elisa kit
Human Cytochrome B-c1 Complex Subunit 10 (UQCR) ELISA Kit
Gene Names
UQCR11; UQCR; QCR10; 0710008D09Rik
Reactivity
Human
Synonyms
Cytochrome B-c1 Complex Subunit 10 (UQCR); N/A; Human Cytochrome B-c1 Complex Subunit 10 (UQCR) ELISA Kit; UQCR; 0710008D09Rik; QCR10; UQCR11; complexI subunit XI; cytochrome b-c1 complex subunit 10; ubiquinol cytochrome c reductase; 6.4kDa subunit; UQCR elisa kit
Reactivity
Human
Specificity
Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit has high sensitivity and excellent specificity for detection of Human UQCR. No significant cross-reactivity or interference between Human UQCR and analogues was observed.
Sequence Length
56
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Cytochrome b-c1 complex subunit 10 (UQCR) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Cytochrome b-c1 complex subunit 10 (UQCR) Standard Curve.)
Related Product Information for UQCR elisa kit
Description: This Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit employs a two-site sandwich ELISA to quantitate UQCR in samples. An antibody specific for UQCR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UQCR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: UQCRB is a subunit of the respiratory chain protein Ubiquinol Cytochrome c Reductase (UQCR,Complex III or Cytochrome bc1 complex), Subunit 7 was identified as a Q-binding protein by photo-labeling with a ubiquinone analog (subsequent structures show it to be exposed to the lipid phase but not involved in either Q-binding site). Subunits 6 and 7 reverse position on going from Laemli gels to Weber&Osborne gels, and one might suspect the name "Q-binding protein" arose from confusion with subunit 7. I have been told however that this is not the case, and both proteins were separately identified as Q-binding proteins. Genome anotators improved the situation by naming its gene "UQCR binding", UQCRB.
Background: UQCRB is a subunit of the respiratory chain protein Ubiquinol Cytochrome c Reductase (UQCR,Complex III or Cytochrome bc1 complex), Subunit 7 was identified as a Q-binding protein by photo-labeling with a ubiquinone analog (subsequent structures show it to be exposed to the lipid phase but not involved in either Q-binding site). Subunits 6 and 7 reverse position on going from Laemli gels to Weber&Osborne gels, and one might suspect the name "Q-binding protein" arose from confusion with subunit 7. I have been told however that this is not the case, and both proteins were separately identified as Q-binding proteins. Genome anotators improved the situation by naming its gene "UQCR binding", UQCRB.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cytochrome b-c1 complex subunit 10
NCBI Official Synonym Full Names
ubiquinol-cytochrome c reductase, complex III subunit XI
NCBI Official Symbol
UQCR11
NCBI Official Synonym Symbols
UQCR; QCR10; 0710008D09Rik
NCBI Protein Information
cytochrome b-c1 complex subunit 10
UniProt Protein Name
Cytochrome b-c1 complex subunit 10
UniProt Gene Name
UQCR11
UniProt Synonym Gene Names
UQCR
UniProt Entry Name
QCR10_HUMAN
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Product Notes
The Human UQCR uqcr11 (Catalog #AAA330309) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330309 ELISA Kit recognizes Human UQCR. It is sometimes possible for the material contained within the vial of "Cytochrome B-c1 Complex Subunit 10 (UQCR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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