WB (Western Blot)
(Western blot All lanes: Cytochrome b-c1 complex subunit 10 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 6.4kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Rabbit anti-Human Cytochrome b-c1 complex subunit 10 Polyclonal Antibody | anti-UQCR11 antibody
Rabbit anti- human Cytochrome b-c1 complex subunit 10 polyclonal Antibody, FITC
Gene Names
UQCR11; UQCR; QCR10; 0710008D09Rik
Reactivity
Human
Applications
ELISA
Purity
>95%, Protein G purified
Synonyms
Cytochrome b-c1 complex subunit 10, Antibody; Rabbit anti- human Cytochrome b-c1 complex subunit 10 polyclonal Antibody, FITC; Cytochrome b-c1 complex subunit 10 (Complex III subunit 10) (Complex III subunit XI) (Ubiquinol-cytochrome c reductase complex 6.4 kDa protein); UQCR11; UQCR; anti-UQCR11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
56
Applicable Applications for anti-UQCR11 antibody
ELISA
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
FITC
Immunogen
Recombinant Human Cytochrome b-c1 complex subunit 10 protein (1-56AA)
Immunogen Species
Homo sapiens (Human)
Research Area
Others
Target Names
UQCR11
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
WB (Western Blot)
(Western blot All lanes: Cytochrome b-c1 complex subunit 10 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 6.4kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
WB (Western Blot)
(Western blot All lanes: Cytochrome b-c1 complex subunit 10 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 6.4kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Related Product Information for anti-UQCR11 antibody
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may be closely linked to the iron-sulfur protein in the complex and function as an iron-sulfur protein binding factor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cytochrome b-c1 complex subunit 10
NCBI Official Synonym Full Names
ubiquinol-cytochrome c reductase, complex III subunit XI
NCBI Official Symbol
UQCR11
NCBI Official Synonym Symbols
UQCR; QCR10; 0710008D09Rik
NCBI Protein Information
cytochrome b-c1 complex subunit 10
UniProt Protein Name
Cytochrome b-c1 complex subunit 10
UniProt Gene Name
UQCR11
UniProt Synonym Gene Names
UQCR
UniProt Entry Name
QCR10_HUMAN
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Product Notes
The UQCR11 uqcr11 (Catalog #AAA81433) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti- human Cytochrome b-c1 complex subunit 10 polyclonal Antibody, FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome b-c1 complex subunit 10 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the UQCR11 uqcr11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome b-c1 complex subunit 10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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