Standard Curve (Sample)
(Fig.1. Human DNA topoisomerase 3-alpha (TOP3A) Standard Curve.)
Human DNA Topoisomerase 3-alpha (TOP3A) ELISA Kit | TOP3A elisa kit
Human DNA Topoisomerase 3-alpha (TOP3A) ELISA Kit
Gene Names
TOP3A; TOP3; PEOB5; ZGRF7; MGRISCE2
Reactivity
Human
Synonyms
DNA Topoisomerase 3-alpha (TOP3A); N/A; Human DNA Topoisomerase 3-alpha (TOP3A) ELISA Kit; TOP3A; TOP3; topoI-alpha; TOP3A elisa kit
Reactivity
Human
Specificity
Human DNA topoisomerase 3-alpha (TOP3A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOP3A. No significant cross-reactivity or interference between Human TOP3A and analogues was observed.
Sequence Length
1001
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human DNA topoisomerase 3-alpha (TOP3A) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human DNA topoisomerase 3-alpha (TOP3A) Standard Curve.)
Related Product Information for TOP3A elisa kit
Description: This Human DNA topoisomerase 3-alpha (TOP3A) ELISA Kit employs a two-site sandwich ELISA to quantitate TOP3A in samples. An antibody specific for TOP3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOP3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOP3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOP3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DNA topoisomerase 3-alpha is ana DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells.High expression is found in testis, heart, skeletal muscle and pancreas. Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.
Background: DNA topoisomerase 3-alpha is ana DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells.High expression is found in testis, heart, skeletal muscle and pancreas. Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA topoisomerase 3-alpha isoform 1
NCBI Official Synonym Full Names
DNA topoisomerase III alpha
NCBI Official Symbol
TOP3A
NCBI Official Synonym Symbols
TOP3; PEOB5; ZGRF7; MGRISCE2
NCBI Protein Information
DNA topoisomerase 3-alpha
UniProt Protein Name
DNA topoisomerase 3-alpha
UniProt Gene Name
TOP3A
UniProt Synonym Gene Names
TOP3
UniProt Entry Name
TOP3A_HUMAN
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Product Notes
The Human TOP3A top3a (Catalog #AAA330379) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330379 ELISA Kit recognizes Human TOP3A. It is sometimes possible for the material contained within the vial of "DNA Topoisomerase 3-alpha (TOP3A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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