Rabbit DNA Polyclonal Antibody | anti-DNA antibody

Rabbit anti-Human DNA-(apurinic or apyrimidinic site) lyase polyclonal Antibody

Cat. Num. AAA117882

• Gene Names: APEX1; APE; APX; APE1; APEN; APEX; HAP1; REF1
• Reactivity: Human. Other species are not tested. Please decide the specificity by homology.
• Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: DNA, Antibody; Rabbit anti-Human DNA-(apurinic or apyrimidinic site) lyase polyclonal Antibody; APEX nuclease; Apurinic-apyrimidinic endonuclease 1; REF-1; Redox factor-1; APEX1; APE; APE1; APEX; APX; HAP1; REF1; anti-DNA antibody

• Host: Rabbit
• Reactivity: Human. Other species are not tested. Please decide the specificity by homology.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 318

• Applicable Applications for anti-DNA antibody: ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant Human DNA-(apurinic or apyrimidinic site) lyase Protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9919 / sc-9904 / sc-55498 / sc-5572 / sc-334
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

ChIP (Chromatin immunoprecipitation)

(Chromatin Immunoprecipitation MCF7(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-APE1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the MDR1 promoter.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human tonsil using AAA117882 at dilution of 1:100)

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded human testis using AAA117882 at dilution of 1:100)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human lungs using AAA117882 at dilution of 1:100)

WB (Western Blot)

(Western blotAll lanes: DNA-(apurinic or apyrimidinic site) lyase antibody at 2ug/mlLane 1: Hela whole cell lysateLane 2: Mouse brain tissueLane 3: MCF-7 whole cell lysateLane 4: HepG2 whole cell lysateLane 5: Raji whole cell lysateLane 6: A549 whole cell lysateLane 7: K562 whole cell lysateLane 8: Mouse liver tissueLane 9:NIH3T3 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 36 kDaObserved band size: 36;34;21 kDa)

Related Product Information for anti-DNA antibody

Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.

NCBI and Uniprot Product Information

• NCBI GI #: 346644849
• NCBI GeneID: 328
• NCBI Accession #: NP_001231178.1
• NCBI GenBank Nucleotide #: NM_001244249.1
• UniProt Accession #: P27695; Q969L5; Q99775
• Molecular Weight: 35,555 Da
• NCBI Official Full Name: DNA-(apurinic or apyrimidinic site) lyase
• NCBI Official Synonym Full Names: APEX nuclease (multifunctional DNA repair enzyme) 1
• NCBI Official Symbol: APEX1
• NCBI Official Synonym Symbols: APE; APX; APE1; APEN; APEX; HAP1; REF1
• NCBI Protein Information: DNA-(apurinic or apyrimidinic site) lyase
• UniProt Protein Name: DNA-(apurinic or apyrimidinic site) lyase
• UniProt Gene Name: APEX1
• UniProt Synonym Gene Names: APE; APE1; APEX; APX; HAP1; REF1; APEN; AP endonuclease 1; APE-1
• UniProt Entry Name: APEX1_HUMAN