Standard Curve (Sample)
Human HE4 (epididymal protein 4) ELISA Kit | HE4 elisa kit
Uncoated Human HE4 (epididymal protein 4) ELISA Kit
Reactivity
Human
Synonyms
HE4 (epididymal protein 4); N/A; Uncoated Human HE4 (epididymal protein 4) ELISA Kit; HE4 elisa kit
Reactivity
Human
Samples
Serum, plasma
Assay Type
SandwichDetection Range
Detection Type
Colormetric
Preparation and Storage
-20 degree C. Please refer to the instructions for details.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HE4 elisa kit
The new series of Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
Test Principle: 1. Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked.
2. Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted.
3. The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away.
4. HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away.
5. TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured.
Test Principle: 1. Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked.
2. Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted.
3. The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away.
4. HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away.
5. TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured.
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Product Notes
The Human HE4 (Catalog #AAA178500) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178500 ELISA Kit recognizes Human HE4. It is sometimes possible for the material contained within the vial of "HE4 (epididymal protein 4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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