Standard Curve (Sample)
Human Lipocalin-2 protein (NGAL) ELISA Kit | LCN2 elisa kit
Lipocalin-2 protein (NGAL): Human (ELISA)
Gene Names
LCN2; p25; 24p3; MSFI; NGAL
Reactivity
Human
Synonyms
Lipocalin-2 protein (NGAL); N/A; Lipocalin-2 protein (NGAL): Human (ELISA); Human lipocalin 2 (NGAL); LCN2 elisa kit
Reactivity
Human
Specificity
The antibody pair used in this assay is specific to human lipocalin-2 and does not cross-react with mouse and rat lipocalin-2, and other cytokine or hormone molecules.
Sequence Length
198
Samples
Serum, plasma, urine and cell culture supernate samples
Assay Type
Quantitative Sandwich
Sensitivity
0.39 ng/ml
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LCN2 elisa kit
Intended Uses: This Human lipocalin-2 ELISA kit is designed for quantification of human lipocalin-2 in serum, plasma, urine and cell culture supernate samples.
Principle of the Assay: This assay is a quantitative sandwich ELISA using monoclonal antibodies against human LCN2. The immunoplate is pre-coated with a monoclonal antibody specific for human LCN2. Standards and samples are added into the wells and any human LCN2 present is captured by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked monoclonal antibody specific for human LCN2 is added to the wells. After a final washing step to remove any unbound reagents, a HRP substrate solution is added and colour develops in proportion to the amount of human LCN2 bound initially. The reaction is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human LCN2, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Lipocalin-2(LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), 24p3, or neutrophil lipocalin (NL), is a 25-kDa secretory glycoprotein1. LCN2 has been implicated in a variety of cellular processes including the innate immune response, differentiation, tumorigenesis, and cell survival2,3. It appears to be upregulated in various inflammation and infection conditions. Several reports suggest that LCN2 may represent a sensitive biomarker for various renal injuries4 and is associated with several types of cancers, including breast cancer5, ovarian, colorectal, and pancreatic cancers6,7. Furthermore, a growing body of evidence suggests that serum levels of lipocalin-2 are correlated with obesity, insulin resistance, hyperglycemia, coronary heart disease and fatty liver disease in humans8-11.
Principle of the Assay: This assay is a quantitative sandwich ELISA using monoclonal antibodies against human LCN2. The immunoplate is pre-coated with a monoclonal antibody specific for human LCN2. Standards and samples are added into the wells and any human LCN2 present is captured by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked monoclonal antibody specific for human LCN2 is added to the wells. After a final washing step to remove any unbound reagents, a HRP substrate solution is added and colour develops in proportion to the amount of human LCN2 bound initially. The reaction is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human LCN2, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Lipocalin-2(LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), 24p3, or neutrophil lipocalin (NL), is a 25-kDa secretory glycoprotein1. LCN2 has been implicated in a variety of cellular processes including the innate immune response, differentiation, tumorigenesis, and cell survival2,3. It appears to be upregulated in various inflammation and infection conditions. Several reports suggest that LCN2 may represent a sensitive biomarker for various renal injuries4 and is associated with several types of cancers, including breast cancer5, ovarian, colorectal, and pancreatic cancers6,7. Furthermore, a growing body of evidence suggests that serum levels of lipocalin-2 are correlated with obesity, insulin resistance, hyperglycemia, coronary heart disease and fatty liver disease in humans8-11.
References
1. A emerging clinical and experimental evidence for the role of lipocalin-2 in metabolic syndrome. Jang Y, Lee JH, Wang Y, Sweeney G. Clin Exp Pharmacol Physiol. 2012 Feb;39(2):194-9
2. The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer. Chakraborty S, Kaur S, Guha S, Batra SK. Biochim Biophys Acta. 2012 Aug;1826(1):129-69.
2. The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer. Chakraborty S, Kaur S, Guha S, Batra SK. Biochim Biophys Acta. 2012 Aug;1826(1):129-69.
NCBI and Uniprot Product Information
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Product Notes
The Human LCN2 (Catalog #AAA60540) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA60540 ELISA Kit recognizes Human LCN2. It is sometimes possible for the material contained within the vial of "Lipocalin-2 protein (NGAL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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