Human MMP-9 ELISA Kit | MMP-9 elisa kit

Human MMP-9 ELISA KIT

Cat. Num. AAA309890

• Gene Names: MMP9; GELB; CLG4B; MMP-9; MANDP2
• Reactivity: Human
• Synonyms: MMP-9; N/A; Human MMP-9 ELISA KIT; Human MMP-9; MMP-9 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MMP-9 Ligand
• Assay Type: Solid Phase Sandwich ELISA
• Samples: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 31pg/mL

• Assay Time: 3 hours
• Sample Volume: 20 uL
• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C

Standard Curve (Sample)

(Representative standard curve for MMP-9 ELISA. MMP-9 was diluted in serial two-fold steps in Sample Diluent.)

Related Product Information for MMP-9 elisa kit

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade extracellular matrix proteins (1 - 3). They are secreted as zymogens (Pro-MMPs) that are activated by a variety of proteinases or by reaction with organic mercurials. They are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs) and by 2-macroglobulin (3 - 6). The regulation of MMP activity is important in tissue remodeling, inflammation, tumor growth and metastasis (3, 7 - 9).Human MMP-9 (also known as gelatinase B) is secreted as a 92 kDa zymogen (2, 3). Cleavage of Pro-MMP-9 at or near residue 87 results in the active enzyme with a mass of approximately 82 kDa (1). MMP-9 has three fibronectin type II domains, a hemopexin-like domain and a proline-rich type V collagen-homologous domain (1 - 3). Pro-MMP-9 can be activated by MMP-3 (5) or by certain bacterial proteinases (10). MMP-9 is inhibited bya2-macroglobulin or by TIMP-1 (3 - 6), which binds to Pro-MMP-9 as well as to active MMP-9 (3). In vitro treatment of Pro-MMP-9 with 4-aminophenylmercuric acid (APMA) produces not only the 82 kDa active enzyme but also a C-terminal truncated form of approximately 65 kDa with the activity comparable to that of the 82 kDa form (11).Pro-MMP-9 is secreted by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, chondrocytes, skeletal muscle satellite cells, endothelial cells, and various tumor cells (1, 7- 21). Pro-MMP-9 expression is upregulated by TGF-b1, IL-1b, TGF-a, PDGF-AB, TNF-a, and IL-1a (7, 15, 17). Substrates for MMP-9 include denatured collagen type I (gelatin), native collagens type IV, V, VII, X and XI, fibrinogen, vitronectin, IL-1b, and entactin, a molecule that bridges laminin and type IV collagen (3, 4, 6, 13, 21 - 23).

NCBI and Uniprot Product Information

• NCBI GI #: 74272287
• NCBI GeneID: 4318
• NCBI Accession #: NP_004985.2
• NCBI GenBank Nucleotide #: NM_004994.2
• UniProt Accession #: P14780; Q3LR70; Q8N725; Q9H4Z1; Q9UCJ9; Q9UCL1; Q9UDK2; B2R7V9
• Molecular Weight: 78,458 Da
• NCBI Official Full Name: matrix metalloproteinase-9 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 9
• NCBI Official Symbol: MMP9
• NCBI Official Synonym Symbols: GELB; CLG4B; MMP-9; MANDP2
• NCBI Protein Information: matrix metalloproteinase-9
• UniProt Protein Name: Matrix metalloproteinase-9
• UniProt Gene Name: MMP9
• UniProt Synonym Gene Names: CLG4B; MMP-9; GELB