Human RAGE ELISA Kit | RAGE elisa kit

Human RAGE PicoKine Quick ELISA Kit

Cat. Num. AAA128075

• Reactivity: Human
• Applications: ELISA
• Synonyms: RAGE; N/A; Human RAGE PicoKine Quick ELISA Kit; Advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor; cDNA FLJ56412, highly similar to Advanced glycosylation end product-specific receptor; RAGE elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Assay Range: 78pg/ml-5,000pg/ml
• Sensitivity: <10pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q24-A344
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for RAGE elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
  O.D.	0.037	0.116	0.191	0.330	0.611	1.112	1.761	2.023

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	154	623	3076	159	595	3344
  Standard deviation	10.16	27.41	221.47	10.65	32.72	250.8
  CV (%)	6.6%	4.4%	7.2%	6.7%	5.5%	7.5%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	154	153	141	152	150	5.24	3.4%
  Sample 2	623	570	627	596	604	22.96	3.8%
  Sample 3	3076	3035	3093	3144	3087	39.08	1.2%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Human Rage PicoKine ELISA Kit standard curve)

Related Product Information for RAGE elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for RAGE elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GeneID: 177
• UniProt Accession #: B4DNX3
• Molecular Weight: Calculated Molecular Weight: 45980
• UniProt Protein Name: cDNA FLJ56412, highly similar to Advanced glycosylation end product-specific receptor
• UniProt Entry Name: B4DNX3_HUMAN