ELISA
(Sandwich ELISA analysis of human RAGE using Mouse anti Human RAGE as a capture reagent and biotinylated Mouse anti Human RAGE (AAA50429) as a detection reagent with purified recombinant RAGE as the antigen. Detection was performed using HRP co)
Mouse anti-Human RAGE Monoclonal Antibody | anti-RAGE antibody
Mouse anti Human RAGE:Biotin
Gene Names
AGER; RAGE
Reactivity
Human
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
RAGE, Antibody; Mouse anti Human RAGE:Biotin; RAGE antibody | A09-5H8; anti-RAGE antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
A09-5H8
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-RAGE antibody
ELISA
Immunogen
E Coli derived recombinant human full length RAGE (Ala23-Pro404)
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Conjugation
Biotin
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA
(Sandwich ELISA analysis of human RAGE using Mouse anti Human RAGE as a capture reagent and biotinylated Mouse anti Human RAGE (AAA50429) as a detection reagent with purified recombinant RAGE as the antigen. Detection was performed using HRP co)
ELISA
(Sandwich ELISA analysis of human RAGE using Mouse anti Human RAGE as a capture reagent and biotinylated Mouse anti Human RAGE (AAA50429) as a detection reagent with purified recombinant RAGE as the antigen. Detection was performed using HRP co)
Related Product Information for anti-RAGE antibody
Mouse anti Human RAGE antibody, clone A09-5H8, recognizes Receptor for Advanced Glycosylation End products (RAGE), also known as advanced glycosylation end product-specific receptor.
RAGE is a multi-ligand receptor belonging to the immunoglobulin receptor subfamily. It has many endogenous and exogenous ligands and upon binding of a ligand, RAGE can stimulate various signaling pathways including NF-kappaB, AP-1, CREB and STAT3. Triggering these pathways can then induce cytokine/chemokine transcription (Sorci et al. 2013). This can be used to regulate the innate immune response, inflammation resolution, tissue homeostasis and tissue repair following injury (Plotkin et al. 2019).
The biotinylated Mouse anti Human RAGE antibody, clone A09-5H8 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human RAGE antibody, clone B02-4G7 as the capture antibody.
RAGE is a multi-ligand receptor belonging to the immunoglobulin receptor subfamily. It has many endogenous and exogenous ligands and upon binding of a ligand, RAGE can stimulate various signaling pathways including NF-kappaB, AP-1, CREB and STAT3. Triggering these pathways can then induce cytokine/chemokine transcription (Sorci et al. 2013). This can be used to regulate the innate immune response, inflammation resolution, tissue homeostasis and tissue repair following injury (Plotkin et al. 2019).
The biotinylated Mouse anti Human RAGE antibody, clone A09-5H8 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human RAGE antibody, clone B02-4G7 as the capture antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,803 Da
NCBI Official Full Name
advanced glycosylation end product-specific receptor isoform 6
NCBI Official Synonym Full Names
advanced glycosylation end product-specific receptor
NCBI Official Symbol
AGER
NCBI Official Synonym Symbols
RAGE
NCBI Protein Information
advanced glycosylation end product-specific receptor; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta
UniProt Protein Name
Advanced glycosylation end product-specific receptor
UniProt Gene Name
AGER
UniProt Synonym Gene Names
RAGE
UniProt Entry Name
RAGE_HUMAN
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Product Notes
The RAGE ager (Catalog #AAA50429) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human RAGE:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAGE can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the RAGE ager for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAGE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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