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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with RAGE antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit RAGE Monoclonal Antibody | anti-RAGE antibody
RAGE Antibody
Gene Names
AGER; RAGE
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
RAGE, Antibody; RAGE Antibody; Advanced glycosylation end product-specific receptor antibody; Ager antibody; MGC2235 antibody; RAGE_HUMAN antibody; Receptor for advanced glycosylation end products antibody; anti-RAGE antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JF0975
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RAGE antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with RAGE antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with RAGE antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining RAGE in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RAGE in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RAGE in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RAGE in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RAGE antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RAGE antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1, 000 dilution.)
Related Product Information for anti-RAGE antibody
Advanced glycosylation end products of proteins (AGEs) are nonenzymatically glycosylated proteins that are associated with a variety of conditions, including diabetes and other vascular disorders, as well as amyloidosis. These proteins regulate cellular functions via specific cell surface acceptor molecules, such as RAGE (receptor for advanced glycosylation end products). RAGE is a type 1 membrane protein that is found on the surface of endothelial cells, mononuclear phagocytes and vascular smooth muscle cells. Binding of AGEs to RAGE results in the induction of cellular oxidant stress and activation of the transcription factor NFkB. Evidence suggests that the induction of oxidant stress results in the activation of an intracellular cascade involving p21 ras and MAP kinase, which leads to activation of transcription.
Product Categories/Family for anti-RAGE antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,803 Da
NCBI Official Full Name
advanced glycosylation end product-specific receptor isoform 6
NCBI Official Synonym Full Names
advanced glycosylation end product-specific receptor
NCBI Official Symbol
AGER
NCBI Official Synonym Symbols
RAGE
NCBI Protein Information
advanced glycosylation end product-specific receptor; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta
UniProt Protein Name
Advanced glycosylation end product-specific receptor
UniProt Gene Name
AGER
UniProt Synonym Gene Names
RAGE
UniProt Entry Name
RAGE_HUMAN
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Product Notes
The RAGE ager (Catalog #AAA312410) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAGE Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAGE can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RAGE ager for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAGE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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