Standard Curve (Sample)
(Fig.1. Human Receptor for advanced glycation end products (RAGE/AGER) Standard Curve.)
Human Receptor For Advanced Glycation End Products (RAGE/AGER) ELISA Kit | RAGE/AGER elisa kit
Human Receptor For Advanced Glycation End Products (RAGE/AGER) ELISA Kit
Gene Names
AGER; RAGE; SCARJ1
Reactivity
Human
Synonyms
Receptor For Advanced Glycation End Products (RAGE/AGER); N/A; Human Receptor For Advanced Glycation End Products (RAGE/AGER) ELISA Kit; AGER; DAMA-358M23.4; MGC22357; RAGE; receptor for advanced glycosylation end-products; RAGE/AGER elisa kit
Reactivity
Human
Specificity
Human Receptor for advanced glycation end products (RAGE/AGER) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGER. No significant cross-reactivity or interference between Human AGER and analogues was observed.
Sequence Length
347
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Receptor for advanced glycation end products (RAGE/AGER) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Receptor for advanced glycation end products (RAGE/AGER) Standard Curve.)
Related Product Information for RAGE/AGER elisa kit
Description: This Human Receptor for advanced glycation end products (RAGE/AGER) ELISA Kit employs a two-site sandwich ELISA to quantitate AGER in samples. An antibody specific for AGER has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGER present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGER is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGER bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RAGE, the receptor for advanced glycation endproducts is a 35kD transmembrane receptor of the immunoglobulin super family which was first characterized in 1992 by Neeper et al.. Its name comes from its ability to bind advanced glycation endproducts (AGE), a heterogeneous group of non-enzymatically altered proteins. Besides AGEs, RAGE is also able to bind other ligands and is thus often referred to as a pattern recognition receptor.The interaction between RAGE and its ligands is thought to result in pro-inflammatory gene activation. Due to an enhanced level of RAGE ligands in diabetes or other chronic disorders, this receptor is hypothesised to have a causative effect in a range of inflammatory diseases such as diabetic complications, Alzheimer's disease and even some tumors.
Background: RAGE, the receptor for advanced glycation endproducts is a 35kD transmembrane receptor of the immunoglobulin super family which was first characterized in 1992 by Neeper et al.. Its name comes from its ability to bind advanced glycation endproducts (AGE), a heterogeneous group of non-enzymatically altered proteins. Besides AGEs, RAGE is also able to bind other ligands and is thus often referred to as a pattern recognition receptor.The interaction between RAGE and its ligands is thought to result in pro-inflammatory gene activation. Due to an enhanced level of RAGE ligands in diabetes or other chronic disorders, this receptor is hypothesised to have a causative effect in a range of inflammatory diseases such as diabetic complications, Alzheimer's disease and even some tumors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
advanced glycosylation end product-specific receptor isoform 6
NCBI Official Synonym Full Names
advanced glycosylation end-product specific receptor
NCBI Official Symbol
AGER
NCBI Official Synonym Symbols
RAGE; SCARJ1
NCBI Protein Information
advanced glycosylation end product-specific receptor
UniProt Protein Name
Advanced glycosylation end product-specific receptor
UniProt Gene Name
AGER
UniProt Synonym Gene Names
RAGE
UniProt Entry Name
RAGE_HUMAN
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Product Notes
The Human RAGE/AGER ager (Catalog #AAA331145) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331145 ELISA Kit recognizes Human RAGE/AGER. It is sometimes possible for the material contained within the vial of "Receptor For Advanced Glycation End Products (RAGE/AGER), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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