Human soluble CD5 ELISA Kit | CD5 elisa kit

Human soluble CD5 PicoKine ELISA Kit

Cat. Num. AAA45843

• Gene Names: CD5; T1; LEU1
• Reactivity: Human
• Synonyms: soluble CD5; N/A; Human soluble CD5 PicoKine ELISA Kit; T-cell surface glycoprotein CD5; CD5 molecule; CD 5; CD5; CD5 antigen (p56 62); CD5 antigen; CD5_HUMAN; LEU 1; LEU1; Ly12; LyA; Lymphocyte Antigen CD5; Lymphocyte antigen T1/Leu 1; Lymphocyte antigen T1/Leu-1; Lymphocyte glycoprotein T1/Leu1; OTTHUMP00000236973; p56 62; T cell surface glycoprotein CD5; T1; CD5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CD5
• Sequence Length: 495
• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <10 pg/ml

• Intra-assay Precision: Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

Related Product Information for CD5 elisa kit

Background: CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a Tcell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins.

Principle of the Assay: The Picokine™ Human CD5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CD5 with a 96-well strip plate that is pre-coated with antibody specific for CD5. The detection antibody is a biotinylated antibody specific for CD5. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CD5 with immunogen: Expression system for standard: NS0; Immunogen sequence: R25-N371. The kit is analytically validated with ready-to-use reagents. To measure Human CD5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CD5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD5 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 313104090
• NCBI GeneID: 921
• UniProt Accession #: P06127; A0N0P4; A8K9I3
• Molecular Weight: 54,578 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD5
• NCBI Official Synonym Full Names: CD5 molecule
• NCBI Official Symbol: CD5
• NCBI Official Synonym Symbols: T1; LEU1
• NCBI Protein Information: T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1
• UniProt Protein Name: T-cell surface glycoprotein CD5
• UniProt Gene Name: CD5
• UniProt Synonym Gene Names: LEU1
• UniProt Entry Name: CD5_HUMAN