Human T cell surface glycoprotein CD3 epsilon chain (CD3E) ELISA Kit | CD3E elisa kit

Human T cell surface glycoprotein CD3 epsilon chain (CD3E) ELISA Kit

Cat. Num. AAA246613

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human
• Synonyms: T cell surface glycoprotein CD3 epsilon chain (CD3E); N/A; Human T cell surface glycoprotein CD3 epsilon chain (CD3E) ELISA Kit; CD3E elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CD3E. No significant cross-reactivity or interference between CD3E and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD3E and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CD3E elisa kit

Principle of the Assay: CD3E ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CD3E antibody and an CD3E-HRP conjugate. The assay sample and buffer are incubated together with CD3E-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD3E concentration since CD3E from samples and CD3E-HRP conjugate compete for the anti-CD3E antibody binding site. Since the number of sites is limited, as more sites are occupied by CD3E from the sample, fewer sites are left to bind CD3E-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD3E concentration in each sample is interpolated from this standard curve.

Intended Uses: This CD3E ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD3E. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for CD3E elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724.1
• NCBI GenBank Nucleotide #: NM_000733.3
• Molecular Weight: 23,147 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule, epsilon (CD3-TCR complex)
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN