Standard Curve (Sample)
Human TOM20 (translocase of outer mitochondrial membrane 20 homolog) ELISA Kit | TOM20 elisa kit
Human TOM20 (translocase of outer mitochondrial membrane 20 homolog) ELISA Kit
Gene Names
TOMM20; MAS20; MOM19; TOM20
Reactivity
Human
Synonyms
TOM20 (translocase of outer mitochondrial membrane 20 homolog); N/A; Human TOM20 (translocase of outer mitochondrial membrane 20 homolog) ELISA Kit; TOM20 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TOM20. No significant cross-reactivity or interference between TOM20 and analogues was observed.
Sequence Length
145
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TOM20 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TOM20 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TOM20 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TOM20 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TOM20 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TOM20 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TOM20 can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,298 Da
NCBI Official Full Name
Translocase of outer mitochondrial membrane 20 homolog (yeast)
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 20
NCBI Official Symbol
TOMM20
NCBI Official Synonym Symbols
MAS20; MOM19; TOM20
NCBI Protein Information
mitochondrial import receptor subunit TOM20 homolog
UniProt Protein Name
Mitochondrial import receptor subunit TOM20 homolog
UniProt Gene Name
TOMM20
UniProt Synonym Gene Names
KIAA0016
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Product Notes
The Human TOM20 tomm20 (Catalog #AAA248788) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA248788 ELISA Kit recognizes Human TOM20. It is sometimes possible for the material contained within the vial of "TOM20 (translocase of outer mitochondrial membrane 20 homolog), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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